FastQCFastQC Report
Tue 19 Sept 2023
SRR17238158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58859767
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length5-38
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC9091911.5446731211151414No Hit
GGGAGAGATCC9014121.5314569627841035No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4000500.6796662990527979No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC3940860.6695337411036643No Hit
GGGAGAAAGCC3867030.6569903683104963No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCG3725170.6328890156836673No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG2889710.4909482567268742No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG2889490.4909108797525481No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG2596630.4411553311109777No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2412880.409937062781781No Hit
GGGAGAAAGCCC2267740.3852784534468171No Hit
CGTCCCCGCTCGGCGGGGTCCCCGC2248190.38195699959192836No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG2202360.3741706962584477No Hit
GCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC2036040.34591370366790614Illumina Paired End PCR Primer 2 (100% over 38bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1855380.31522041193265343No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAG1809870.3074884751072834No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGC1678760.28521349736229845No Hit
ATGCGTGCATTTATCAGA1597760.271451974996775No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1456570.24746445224630262No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1359320.23094213064078217No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1272110.21612555822723528No Hit
GGGAGAAAGCCCC1267660.2153695239738207No Hit
CTCGCTTCTGGCGCCAAGCGTCCG1266420.21515885375489166No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCG1229550.2088948126485108No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC1228500.20871642254377257No Hit
GGGAGAGCGACGCTCAGAC1190870.20232326098062878No Hit
GGGGAGAGATCC1159690.19702592434659144No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1099980.18688147372380867No Hit
TCGCTTCTGGCGCCAAGCGTCCG1088000.18484612757641397No Hit
GGGAGAGATC1036290.17606083965639893No Hit
AGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT1026180.17434319779077617Illumina Paired End PCR Primer 2 (100% over 38bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1006990.17108290625751202No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG972150.16516375268695846No Hit
GGGAGAGCGACGCTCAGACA950290.1614498406016456No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT944660.1604933298495728No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGA918000.15596392014259927No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA864110.14680826038608003No Hit
GGGAGAAAGC825650.14027408569252406No Hit
GGGAGA813880.13827441756607703No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC794030.13490199510983453No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC784940.13335764648881468No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCC775890.13182009368130865No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG774240.1315397663738628No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC765360.13003109577379063No Hit
GGGAGAGCGACCCC763580.12972868207242477No Hit
GTATAGTGGTGAGTATC754980.12826758216694947No Hit
TGCGTGCATTTATCAGA743110.12625092450671782No Hit
GGGAGAGCGACCCCC733180.12456386380190734No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA690240.11726855799480145No Hit
GGGAGAGCGACGCTCAGACAGG688480.11696954220019253No Hit
AGGGAGAGATCC685700.11649723316098073No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT655200.1113154253566787No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGG643560.10933784362415164No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC641030.10890800841940132No Hit
CGTCCCCGCTCGGCGGGGTCCCCG628400.10676223030240672No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC624380.10607925104426594No Hit
TCGACTGCATAATTTGTGGTAGTGGGG621290.10555427445032191No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA598060.10160760575216005No Hit
GGGAGAGCGACGCTCAGACAG594460.10099598253591456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)