FastQCFastQC Report
Tue 19 Sept 2023
SRR17238159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67562082
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length5-38
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC13852642.0503571811182493No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC13079091.9358624857061093No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC11760161.74064499670096No Hit
GGGAGAGATCC9051841.339781092003648No Hit
GGGAGAAAGCC5619800.8317979306795193No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCG4916310.7276729571477683No Hit
GGGAGAAAGCCC4895870.724647591529225No Hit
GCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC4037150.5975467126664332Illumina Paired End PCR Primer 2 (100% over 38bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCG3122410.4621542006357945No Hit
GGGAGAAAGCCCC2895560.4285776746785275No Hit
GGGAGAGCGACGCTCAGAC2850700.42193785561552116No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG2814760.41661830374025477No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2744650.40624118125903813No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAG2663450.3942226055141403No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2642990.39119427965526576No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC2642020.3910507079991999No Hit
CTCGCTTCTGGCGCCAAGCGTCCG2254620.3337108527827784No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG2128130.31498881280775215No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC1893130.28020598891549847No Hit
GGGAGAGCCTC1866720.2762969915580754No Hit
GGGAGAGCGACGCTCAGACA1847230.2734122373552668No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1846200.27325978497820713No Hit
TCGCTTCTGGCGCCAAGCGTCCG1834030.27145847873663814No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1743590.2580722719587002No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGC1742550.25791833946147485No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC1684570.2493366027411648No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1650560.24430271405786458No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC1576000.23326693810294363No Hit
GGGAGAGCGACGCTCAGA1566370.23184158238344402No Hit
GGGAGAGCGACCCC1534860.2271777237415508No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1521990.22527280908838776No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1436820.21266662563773567No Hit
GGGAGAGCGACGCTC1426220.2110976982621702No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1424650.21086531939616665No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1368570.20256480550732586No Hit
GGGAGAGCGACCCCC1352530.2001906927616588No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG1280920.18959155225559804No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1248200.18474859907366384No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1181420.1748643566076013No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1151590.1704491581535335No Hit
GGGAGAAAGCCCCC1131310.16744747445764033No Hit
GGGGAGAGATCC1076520.15933789607016552No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAA1075140.15913363948730888No Hit
GGGAGAGCGACGCTCA1045670.1547717253592037No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCG1005590.14883940373536741No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGA995570.14735632332940835No Hit
GGGAGAGATC980830.14517462620527294No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG969300.14346804765430407No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT958380.1418517564334385No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC941380.13933555215187124No Hit
CGTCCCCGCTCGGCGGGGTCCCCGC938300.13887967514085783No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG931030.13780362778044644No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC917040.13573293966873312No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG916290.13562193065631103No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG909200.1345725254588809No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG881910.13053327752688262No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA879290.1301454860434881No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG873810.12933438019272409No Hit
GGGAGAGCGACGCTCAGACAG870700.12887406282121383No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA858240.1270298330948416No Hit
GGGAGAAAGC838750.12414507889203295No Hit
ATTCAACCCGGCGGCGCGC837370.12394082230917632No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG825530.12218836003307298No Hit
GGGAGA825150.12213211546677914No Hit
GGGAGAGCGACGCTCAGACAGG809980.11988677317552174No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCC805090.11916299441452974No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC779760.11541385003499448No Hit
GGGGAGAGCCTC761530.11271559097305497No Hit
AAGCTATTAATGGTTCGTTTGT714740.10579010871808243No Hit
GGAGAGATCC694270.1027603027390423No Hit
GGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC693890.10270405817274844No Hit
CCCTGGTGGTCTAGTGGTTAGGATT683330.10114105127784546No Hit
AGAGCCACCTTACAAATAAGCGCTCTC682910.1010788862308891No Hit
AGGGAGAGATCC679240.10053568213010369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)