FastQCFastQC Report
Tue 19 Sept 2023
SRR17238161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33415362
Total Bases858.5 Mbp
Sequences flagged as poor quality0
Sequence length5-38
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5510531.6491007938205189No Hit
GGGAGAGATCC4580961.3709143716593584No Hit
GGGAGAAAGCCC3019770.9037071033376803No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2954260.8841023478961563No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2933170.8777908795361845No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG2711350.8114082379236233No Hit
GGGAGAAAGCC2451230.733563802181763No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC1885840.5643631812218584No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCG1699860.5087061453950431No Hit
GGGAGAAAGCCCC1597410.4780465942580542No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCG1325320.39661997377134506No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1318040.3944413350961154No Hit
GGGAGAGCGACGCTCAGAC1134790.3396012887725113No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG1101520.32964479032128996No Hit
CTCGCTTCTGGCGCCAAGCGTCCG1079970.323195660726345No Hit
TCGCTTCTGGCGCCAAGCGTCCG1073250.32118460964151757No Hit
CGTCCCCGCTCGGCGGGGTCCCCGC983880.294439425794639No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG978900.2929490932942759No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC893560.26740994157118514No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA876740.26237632858803084No Hit
GGGAGAGCGACCCC857250.2565436819149228No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGC828740.2480116779821209No Hit
GGGAGAGCGACCCCC760080.2274642423445839No Hit
GGGAGAGCGACGCTCAGACA759170.2271919125101802No Hit
GGGAGAGCCTC755360.22605171836833612No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG723400.2164872551732344No Hit
GCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC717460.21470962966075305Illumina Paired End PCR Primer 2 (100% over 38bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC686400.20541450366451217No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC685520.20515115173673715No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGA681900.20406781767020812No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG662750.19833692060555863No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC639090.19125634491106216No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC635220.19009819495596067No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA622290.1862287171989937No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG615800.18428649673165295No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC612950.18343359560192704No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC595180.17811568224219745No Hit
GGGAGAAAGCCCCC590820.17681089314549397No Hit
GGGAGAGCGACGCTCAGA574360.17188501504188403No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT569700.1704904468788936No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC564740.16900609964961624No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG553470.1656333993927703No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC542760.16242828672632664No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG542150.16224573595820987No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC535410.1602286996022967No Hit
AAGCTATTAATGGTTCGTTTGT530540.15877128609290542No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAG525910.1573856958365437No Hit
CGCTTCTGGCGCCAAGCGTCCG524180.15686796988762236No Hit
CCCCC521390.15603302457115384No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG519950.15560208505297654No Hit
GGGGAGAGATCC514480.15396511341101138No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT484730.1450620226708901No Hit
ACCCC484150.14488844980940205No Hit
GGGAGAGATC475880.1424135402154255No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG472470.1413930514952973No Hit
GGGAGAGCGACGCTC462250.13833457797045562No Hit
CCCTGGTGGTCTAGTGGTTAGGATT459500.1375116031961587No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG457540.13692504662975072No Hit
ACCCCC426620.1276718175311104No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA422600.12646877804286544No Hit
GGGAGAAAGC405250.12127655537593758No Hit
GGGAGAGCGACGCTCAGACAG400570.11987600194186135No Hit
ACCTCTAGCATTACAAGTATTAGAGGC393230.1176794074533743No Hit
ACCCCGAGTGTTACAGCCCTCCGG377920.1130976824371976No Hit
GGGGAGAGCCTC375570.11239441308461659No Hit
AGCTATTAATGGTTCGTTTGT371660.11122429258734351No Hit
GGGAGAGCGACGCTCAGACAGG368290.1102157744093869No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA355770.10646899470967874No Hit
GGGAGAGCGACGCTCA350380.10485596415205677No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG348490.10429035603444906No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAG348160.10419159906153344No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG345560.10341351382037998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)