FastQCFastQC Report
Sat 30 Sept 2023
SRR17248232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118297556
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC12946661.0944148330503125No Hit
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCTC6865230.5803357425237087RNA PCR Primer, Index 37 (97% over 37bp)
AGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4595570.3884754812686071RNA PCR Primer, Index 1 (100% over 33bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCT4084650.34528608520027243RNA PCR Primer, Index 37 (97% over 37bp)
ATCTAGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATC2887580.24409464553942264RNA PCR Primer, Index 37 (97% over 37bp)
ATGCATGTCTAAGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2785500.23546555771617123RNA PCR Primer, Index 1 (100% over 33bp)
ACTATCCAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATC2695300.22784071718269483RNA PCR Primer, Index 37 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCTCGTAT2624240.22183383061607798RNA PCR Primer, Index 37 (97% over 37bp)
CGCCGTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCG2486070.21015396125343452RNA PCR Primer, Index 37 (97% over 37bp)
CGAGCCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCG2484120.20998912268314318RNA PCR Primer, Index 37 (97% over 37bp)
AAGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2251850.19035473564644056RNA PCR Primer, Index 1 (100% over 29bp)
ATGCTAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGA2226740.188232122056689RNA PCR Primer, Index 37 (97% over 37bp)
CGCTTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCG2212350.18701569794053904RNA PCR Primer, Index 37 (97% over 37bp)
AACCCGGTGAGCTCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2125370.17966305237954366RNA PCR Primer, Index 1 (100% over 33bp)
AGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2103070.1777779753962119RNA PCR Primer, Index 1 (100% over 30bp)
ACGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAA2054760.17369420548299408RNA PCR Primer, Index 37 (97% over 37bp)
CGCGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAAT2033220.17187337327577587RNA PCR Primer, Index 37 (97% over 37bp)
CCCTCGCTGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGAT2028930.1715107284211349RNA PCR Primer, Index 37 (97% over 37bp)
AGCAGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAA1975310.16697809040112377RNA PCR Primer, Index 37 (97% over 37bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGC1882410.15912501184724392No Hit
ACGTTCGTGTGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1867640.15787646534303718RNA PCR Primer, Index 3 (100% over 34bp)
CGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCT1785180.15090590713471713RNA PCR Primer, Index 37 (97% over 37bp)
ACGCGGGAGACCGGGGTTCGTTTCCCCGACGGGTGGAATTCTCGGGTGCC1776830.1502000599234696No Hit
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1745580.14755841616880067RNA PCR Primer, Index 3 (100% over 34bp)
CGTCCGAAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATC1695660.1433385487693423RNA PCR Primer, Index 37 (97% over 37bp)
AGGATAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGA1677570.14180935403263953RNA PCR Primer, Index 37 (97% over 37bp)
CGCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAA1663770.14064280415057773RNA PCR Primer, Index 37 (97% over 37bp)
GTCCTCGCGTCGGGGTCGGGGCACGCCGGCCTCGCGGTGGAATTCTCGGG1653610.13978395293305976No Hit
AGCCAAGGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGA1628160.13763259825925736RNA PCR Primer, Index 37 (97% over 37bp)
TACTATCCAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGAT1611970.13626401546283848RNA PCR Primer, Index 37 (97% over 37bp)
ATAAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAAT1552490.13123601640595178RNA PCR Primer, Index 37 (97% over 37bp)
AAAAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAAT1523270.12876597382958613RNA PCR Primer, Index 37 (97% over 37bp)
AGAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGA1492420.12615814311497694RNA PCR Primer, Index 37 (97% over 37bp)
CCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGG1437030.12147588239270132RNA PCR Primer, Index 10 (97% over 36bp)
AACCCGGTGAGCTCCCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1422650.12026030360255287RNA PCR Primer, Index 1 (100% over 30bp)
GGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATC1393010.11775475733412447RNA PCR Primer, Index 37 (97% over 37bp)
AGAGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGA1362830.1152035634616154RNA PCR Primer, Index 37 (97% over 37bp)
ATGTCTAAGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGA1352490.11432949637607054RNA PCR Primer, Index 37 (97% over 37bp)
CGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCT1300590.10994225442831633RNA PCR Primer, Index 37 (97% over 37bp)
AGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAATCTC1270400.10739021522980577RNA PCR Primer, Index 37 (97% over 37bp)
CAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATCGAAT1267920.10718057438143525RNA PCR Primer, Index 37 (97% over 37bp)
AGGAGTGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGAT1255260.10611039166354376RNA PCR Primer, Index 37 (97% over 37bp)
AAACGAGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGATC1246200.10534452630619014RNA PCR Primer, Index 37 (97% over 37bp)
AGTCTAACGCGTGCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1185170.10018550171907185RNA PCR Primer, Index 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)