FastQCFastQC Report
Sat 30 Sept 2023
SRR17248233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162368734
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC20138591.24029974884204No Hit
AGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAG4397200.27081568548782303RNA PCR Primer, Index 31 (97% over 37bp)
AAGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG4329660.26665601765423635RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGCCAGTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAG2990060.1841524489560903RNA PCR Primer, Index 31 (97% over 37bp)
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGATCTC2872070.17688565583075863RNA PCR Primer, Index 38 (97% over 40bp)
ATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2584440.15917103843403743RNA PCR Primer, Index 1 (100% over 32bp)
CGTCCGAAAGCCGCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2546730.1568485469622495RNA PCR Primer, Index 1 (100% over 31bp)
AGATGTCCGGGGCTGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2543500.1566496170377235RNA PCR Primer, Index 1 (100% over 33bp)
AAGTCGGAGGTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA2529700.15579969971312332RNA PCR Primer, Index 7 (100% over 35bp)
ACGCGGGAGACCGGGGTTCGTTTCCCCGACGGGTGGAATTCTCGGGTGCC2490010.15335526358171886No Hit
CGGAATCCGCTAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA2394160.1474520334684632RNA PCR Primer, Index 7 (100% over 35bp)
AATACATGCCGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA2031700.12512877017320342RNA PCR Primer, Index 28 (100% over 36bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCA2016580.12419755640885885No Hit
ACCGCCCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAG1979040.12188553493309863RNA PCR Primer, Index 31 (97% over 37bp)
AAGAGGGACGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCT1919420.1182136457379781RNA PCR Primer, Index 38 (97% over 39bp)
ACCGCCCGTCGCTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1901110.11708596557758466RNA PCR Primer, Index 2 (100% over 34bp)
AGGAGTGGAGCCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1882040.11591147837612628RNA PCR Primer, Index 1 (100% over 33bp)
AGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1861310.11463475474286816RNA PCR Primer, Index 1 (100% over 33bp)
AACCCGGCGGCGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1791080.11030941461919633RNA PCR Primer, Index 7 (100% over 35bp)
AGAACGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCT1781590.10972494248800387RNA PCR Primer, Index 38 (97% over 39bp)
AACCCGGTGAGCTCCCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1766920.10882144341902672RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGGGGCCCGAGGTGGGATCCTGGAATTCTCGGGTGCCAAGGAACTC1766580.10880050342697135RNA PCR Primer, Index 1 (100% over 26bp)
GTCCTCGCGTCGGGGTCGGGGCACGCCGGCCTCGCGGTGGAATTCTCGGG1761180.10846792708256259No Hit
GATTCCGGAGAGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1749330.10773810677122111RNA PCR Primer, Index 1 (100% over 32bp)
AAGGAGTCTAACGCGTGCGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1741620.1072632616572597RNA PCR Primer, Index 1 (100% over 28bp)
AGGGGAATCCGACTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1729630.10652481899624838RNA PCR Primer, Index 1 (100% over 32bp)
AACCCGGTGAGCTCCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1727290.10638070258033791RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGGCCCGAGGTGGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1708580.10522838713517345RNA PCR Primer, Index 1 (100% over 29bp)
ACGCCGGCCTCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1648440.10152447206985059RNA PCR Primer, Index 7 (100% over 35bp)
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1637900.10087533231613421RNA PCR Primer, Index 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)