FastQCFastQC Report
Fri 29 Sept 2023
SRR17248234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93091599
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC17446261.8740960717626087No Hit
ACGCGGGAGACCGGGGTTCGTTTCCCCGACGGGTGGAATTCTCGGGTGCC2728940.2931456790209394No Hit
AGGGCGCGCCGAACCACCTCACCCCGAGTGTTACTGGAATTCTCGGGTGC2631710.2827011275206477No Hit
AACCCGGCGGCGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC2331560.2504586906923792RNA PCR Primer, Index 6 (100% over 35bp)
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGTGGAATTCTCGGGTGCC2039670.21910355197572665No Hit
AAGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1985870.2133242979315459RNA PCR Primer, Index 1 (100% over 29bp)
CCCGGCCGGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCT1906090.20475424425785188RNA PCR Primer, Index 8 (97% over 36bp)
CGCCTGTCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCT1835140.19713271871073995No Hit
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGC1781810.19140395257363663No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCC1629590.1750523159452874No Hit
GGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTGTCAATC1537750.16518676406020266RNA PCR Primer, Index 8 (97% over 38bp)
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTGTCAATCTC1515920.16284176190807506RNA PCR Primer, Index 8 (97% over 38bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGC1473060.15823769446693037No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGC1410520.1515195801932675No Hit
ACCGCCCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTT1406330.15106948587272628RNA PCR Primer, Index 8 (97% over 37bp)
ACCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1283000.13782124421345474RNA PCR Primer, Index 6 (100% over 35bp)
AGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1282870.13780727947319932RNA PCR Primer, Index 1 (100% over 33bp)
GTCCTCGCGTCGGGGTCGGGGCACGCCGGCCTCGCGGTGGAATTCTCGGG1264780.13586403215611326No Hit
GCCTGTCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTC1255380.13485427401456496No Hit
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1230750.13220849284155062RNA PCR Primer, Index 6 (100% over 34bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCA1099890.11815137045825155No Hit
AGGGCGCGCCGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1069130.11484709807165305RNA PCR Primer, Index 6 (100% over 35bp)
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTACTGGAATTCTCGGGT1068560.11478586805668684No Hit
ACGCCGGCCTCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1049800.11277064861674574RNA PCR Primer, Index 6 (100% over 35bp)
CGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTGTCAAT1028600.11049332174431765RNA PCR Primer, Index 8 (97% over 38bp)
GTTCCCCTCTTCCTCCCCGCGTCCGGCTGGAATTCTCGGGTGCCAAGGAA976110.10485478931348037RNA PCR Primer, Index 1 (100% over 23bp)
AGCCAAGGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTT943940.10139905320564963RNA PCR Primer, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)