FastQCFastQC Report
Fri 29 Sept 2023
SRR17248235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111459123
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATCTCGTAT7300480.6549916959242538RNA PCR Primer, Index 15 (97% over 38bp)
AACCCGGCGGCGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAA6484200.5817558783411565RNA PCR Primer, Index 7 (97% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA6257140.5613842843532871No Hit
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATCTC5054800.4535115532893615RNA PCR Primer, Index 15 (97% over 38bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA4068200.36499479724059913No Hit
AGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3489410.3130663427165132RNA PCR Primer, Index 1 (100% over 33bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCTGGAATTCTCGGGTGCCA3421020.3069304609547305No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3186870.2859227593240618No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG3112850.2792817596456416No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2961230.26567856630273323No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGC2909020.26099433780759246No Hit
GACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGA2889870.25927621913910087RNA PCR Primer, Index 1 (100% over 22bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATCT2880320.2584194027796181RNA PCR Primer, Index 15 (97% over 38bp)
GGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATC2716030.2436794698267992RNA PCR Primer, Index 15 (97% over 38bp)
AAGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2708860.2430361846647582RNA PCR Primer, Index 1 (100% over 29bp)
ATGCTAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAA2489600.22336439880295847RNA PCR Primer, Index 15 (97% over 38bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2460310.2207365295705763No Hit
AGGATGGCCGAGCGGTCTAAGGCTGCGTTTGGAATTCTCGGGTGCCAAGG2260410.20280170336527767Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA2224330.1995646421872528No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCTGGAATTCTCGGGTGCCA2197920.19719516364757328No Hit
ACCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAA2197350.19714402382297588RNA PCR Primer, Index 7 (97% over 35bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGG2174680.19511009430784773Illumina Small RNA Adapter 2 (100% over 21bp)
CCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAG2165680.19430262339315194RNA PCR Primer, Index 7 (97% over 36bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2119250.1901369706632269No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC2107570.18908905285393282No Hit
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2021990.18141090164508114RNA PCR Primer, Index 1 (100% over 34bp)
AGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1880490.16871566448625294RNA PCR Primer, Index 1 (100% over 30bp)
CGTCCGAAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCC1853170.16626454166519866RNA PCR Primer, Index 15 (97% over 38bp)
AAAGATTAAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTC1849860.16596757180657162RNA PCR Primer, Index 15 (97% over 38bp)
GCCCGGCTAGCTCAGTCGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACT1755170.15747208059406675RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCTGGAATTCTCGGGTGCCAAGGA1670980.1499186387820403RNA PCR Primer, Index 1 (100% over 22bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATCTCGTATG1620660.14540397917898565RNA PCR Primer, Index 15 (97% over 37bp)
GACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA1537970.1379851158527418RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTG1507990.13529534051689962No Hit
AGCCAAGGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGT1492890.13394058376002116RNA PCR Primer, Index 15 (97% over 37bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1490390.13371628628371676No Hit
CCCGGCCGGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAG1462360.131201462979392RNA PCR Primer, Index 7 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG1436870.1289145259109925No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1428390.128153708871368RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAA1425480.1278926266089497RNA PCR Primer, Index 15 (97% over 38bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1395110.12516786086680406No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCC1383160.1240957189300691No Hit
CGCGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAAT1307960.11734885084283321RNA PCR Primer, Index 15 (97% over 38bp)
ACCGGAATGCCTAAAGGAAAGATCCTGGAATTCTCGGGTGCCAAGGAACT1273780.11428225574679966RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGAATCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAG1272280.11414767726101703RNA PCR Primer, Index 7 (97% over 36bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1271320.11406154703011614No Hit
GGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAATCT1261870.11321370256968558RNA PCR Primer, Index 15 (97% over 38bp)
AGGAGTGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTC1229380.11029873256763378RNA PCR Primer, Index 15 (97% over 38bp)
AAGGAGTCTAACGCGTGCGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1221080.10955406494630322RNA PCR Primer, Index 1 (100% over 28bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC1209190.10848730614899957No Hit
ATCTAGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCC1204860.10809882292004039RNA PCR Primer, Index 15 (97% over 38bp)
CGCTCCCGACGTACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1197170.10740888388292809RNA PCR Primer, Index 1 (100% over 34bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTGGAATTCT1183360.10616986462382268No Hit
GGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAAT1179400.10581457742135654RNA PCR Primer, Index 15 (97% over 38bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGG1175280.1054449351804069Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAACT1172570.10520179671609294RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1171310.10508875078803553No Hit
CGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAAT1165290.10454864246509459RNA PCR Primer, Index 15 (97% over 38bp)
CGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGTCCAAAT1139790.10226080820678986RNA PCR Primer, Index 15 (97% over 38bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCCAAG1123710.10081812683920006No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG1119500.10044040988910347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)