FastQCFastQC Report
Sat 30 Sept 2023
SRR17248236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140064333
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCCGGCGGCGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG18352631.3103000319146203RNA PCR Primer, Index 11 (100% over 35bp)
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG9981940.712668227963503RNA PCR Primer, Index 6 (100% over 34bp)
AGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7438440.531073103385999RNA PCR Primer, Index 1 (100% over 33bp)
CGCTCCCGACGTACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG7235570.5165890448355615RNA PCR Primer, Index 6 (100% over 34bp)
CCCCCGGGCCGCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG5684640.4058592132802289RNA PCR Primer, Index 11 (100% over 35bp)
AAGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG5452060.38925398659486RNA PCR Primer, Index 1 (100% over 29bp)
ATCTAGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTT4995340.35664611346844455RNA PCR Primer, Index 4 (97% over 37bp)
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGATCTC4148970.29621888107659783RNA PCR Primer, Index 4 (97% over 37bp)
CCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGA4024350.28732154102358093RNA PCR Primer, Index 2 (97% over 36bp)
CCCGGCCGGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGA4018180.28688102916250635RNA PCR Primer, Index 2 (97% over 36bp)
AGGGGAATCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGA3897520.2782664163331289RNA PCR Primer, Index 2 (97% over 36bp)
CTCGCTCCCGACGTACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3875210.2766735768484329RNA PCR Primer, Index 1 (100% over 32bp)
CGTCCGAAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTT3671990.2621645297807544RNA PCR Primer, Index 4 (97% over 37bp)
ACCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG3630860.25922802202613565RNA PCR Primer, Index 11 (100% over 35bp)
CCCCCGGGCCGCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2962460.21150709367244838RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGATCTCGTAT2772860.19797045690425696RNA PCR Primer, Index 4 (97% over 37bp)
AAGGAGTCTAACGCGTGCGCGATGGAATTCTCGGGTGCCAAGGAACTCCA2702750.1929648999220951RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGATC2461360.17573067655989194RNA PCR Primer, Index 4 (97% over 37bp)
CCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACT2370210.16922295271273666RNA PCR Primer, Index 4 (97% over 37bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGA2355220.1681527302171924RNA PCR Primer, Index 4 (97% over 37bp)
CGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGATCT2320160.16564959474729374RNA PCR Primer, Index 4 (97% over 37bp)
CACCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2225480.1588898438548235RNA PCR Primer, Index 6 (100% over 34bp)
GGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGAT2209830.15777250015533933RNA PCR Primer, Index 4 (97% over 37bp)
AGCCAAGGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGAC2190740.15640955502925932RNA PCR Primer, Index 4 (97% over 37bp)
AGTCTAACGCGTGCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2168050.15478958515441615RNA PCR Primer, Index 1 (100% over 32bp)
ACCCCGAGTGTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG2156380.15395639659384236RNA PCR Primer, Index 11 (100% over 35bp)
CGCGCGGGCGGGGCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2089700.14919572708064086RNA PCR Primer, Index 1 (100% over 30bp)
CGCGCGGGCGGGGCCGGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2072500.14796771994766147RNA PCR Primer, Index 1 (100% over 29bp)
CGCGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGAT1916940.13686139497055258RNA PCR Primer, Index 4 (97% over 37bp)
CGCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGA1888780.13485089026911656RNA PCR Primer, Index 4 (97% over 37bp)
CGCGCGGGCGGGGCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1882830.13442608547602194RNA PCR Primer, Index 1 (100% over 31bp)
CGTCGCGCCGTCGGGTCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCA1877280.13402983898834545RNA PCR Primer, Index 1 (100% over 28bp)
AAAGTCTTTGGGTTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1854150.13237845497754236RNA PCR Primer, Index 6 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1848320.1319622176760732No Hit
GACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGA1847160.1318793985903606RNA PCR Primer, Index 1 (100% over 22bp)
TTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1846810.13185441007311977RNA PCR Primer, Index 6 (100% over 34bp)
AGGAGTCTAACGCGTGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1785040.12744429375892577RNA PCR Primer, Index 1 (100% over 30bp)
AGGATAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGG1782780.12728293933331336RNA PCR Primer, Index 4 (97% over 37bp)
CCTCCCCGCGTCCGGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1732190.12367102765555597RNA PCR Primer, Index 1 (100% over 31bp)
ACCCGTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGG1710080.12209246732356908RNA PCR Primer, Index 4 (97% over 37bp)
CGTCGCGCCGTCGGGTCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC1703570.1216276809028891RNA PCR Primer, Index 1 (100% over 27bp)
AAAGCTGAAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACT1669560.119199510984713RNA PCR Primer, Index 4 (97% over 37bp)
CGCCGTGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTG1636500.11683916704190495RNA PCR Primer, Index 4 (97% over 37bp)
GGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1630520.11641222037590399RNA PCR Primer, Index 1 (100% over 28bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1618240.11553548039956753No Hit
ACGTTCGTGTGGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1574240.11239406680357375RNA PCR Primer, Index 6 (100% over 34bp)
CGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAACT1553020.11087904870114222RNA PCR Primer, Index 1 (100% over 25bp)
ATGCTAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGG1546000.11037785044105411RNA PCR Primer, Index 4 (97% over 37bp)
CTGGGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGG1514450.10812531410119948RNA PCR Primer, Index 4 (97% over 37bp)
CGCTTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTG1513120.10803035773568422RNA PCR Primer, Index 4 (97% over 37bp)
ACCCCGAGTGTTACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGA1479700.10564431131799985RNA PCR Primer, Index 2 (97% over 36bp)
AGAGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTG1447390.10333751419785077RNA PCR Primer, Index 4 (97% over 37bp)
TTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1447360.10333537232494441RNA PCR Primer, Index 1 (100% over 33bp)
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCTGGAATTCTCGGGTGCCA1441890.10294483749835157No Hit
AAAGTCTTTGGGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1431720.10221874258309573RNA PCR Primer, Index 1 (100% over 33bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGG1430210.10211093498014229Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAGTGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACT1413900.10094647007671824RNA PCR Primer, Index 4 (97% over 37bp)
AGGAAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGACTTGGA1405830.10037030626490757RNA PCR Primer, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph