FastQCFastQC Report
Fri 29 Sept 2023
SRR17248237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17248237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113791471
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG27878472.4499612980660035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA12341511.084572498408075No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA12148361.0675984670239476No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA11770471.0343894754642904No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA10004870.8792284616832134No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTATCTCGTAT7422190.6522624178045823RNA PCR Primer, Index 7 (97% over 36bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA7089500.6230256044409515No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC5173480.45464567375177006No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4722710.41503198425126253No Hit
GACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA4201610.3692376909337959RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA3886870.34157832444225983No Hit
TTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3699090.3250762089190323RNA PCR Primer, Index 1 (100% over 33bp)
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTTGGAATTCTCGGGTGC3687520.32405943675690774No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC3613130.31752203994269484No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3591070.3155834060709172No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACCTGGAATTCTCGGG3575480.31421335611348233No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACCTGGAATTCTCGG3458570.3039392996334497No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGT3282320.28845044107040324No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGG3281000.2883344394062715No Hit
AGCGCCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3278260.2880936480731495RNA PCR Primer, Index 2 (100% over 34bp)
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC3244100.2850916656134975RNA PCR Primer, Index 1 (100% over 24bp)
GACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGA3149520.2767799706183603RNA PCR Primer, Index 1 (100% over 22bp)
CGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTATCTC3142320.27614723426855076RNA PCR Primer, Index 7 (97% over 36bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3063010.26917746761530137No Hit
TTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3041100.2672520157508114RNA PCR Primer, Index 2 (100% over 34bp)
GACCGGGGTTCAATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGG3000570.2636902373816751Illumina Small RNA Adapter 2 (100% over 21bp)
GACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGG2944920.2587997126779388Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCT2812300.24714506063464106No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA2762940.2428073014365022No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG2671330.23475661018566144No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGC2651170.2329849484061947No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC2502540.2199233367850566No Hit
GACCGGGGTTCAATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAG2495650.2193178432503083No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA2471480.21719378247601703No Hit
CGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAACT2465750.21669022979762692RNA PCR Primer, Index 1 (100% over 25bp)
GGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCC2415880.2123076517747099RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG2400240.21093320781484579No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGG2378760.20904554437124728No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG2357040.20713678971598848No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTGGAATTCT2290330.20127431167490575No Hit
GGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTATC2273980.19983747288054654RNA PCR Primer, Index 7 (97% over 36bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTC2220770.19516137549535675No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG2186010.19210666500655396No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTGGAATTC2163110.19009421189396522No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGTGGAATTCTCGGGTGCC2152720.18918113818917062No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGC2132120.1873708091883266No Hit
GACCGGGGTTCATTTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGG2114260.18580127152060458Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGGCGGCGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC2065370.18150481594530052RNA PCR Primer, Index 16 (100% over 35bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA2060670.1810917797169526No Hit
CCGAAGGAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCG2031930.17856610712062945RNA PCR Primer, Index 7 (97% over 36bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGCC1996740.17547360821093524No Hit
GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTGGAATTCTCGGGTGCCA1964530.17264299184602333No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGG1948780.17125888108081494No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1930700.16967000980240426No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGG1929320.16954873533535744No Hit
GGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1889590.16605726100508886RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1869990.16433481205282952No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACCTGGAATTCTCGGG1840400.1617344414152094No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACCTGGAATTCTCGG1827060.16056212156709004No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAATGGAATTCTCGGGT1817070.15968419988172927No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGATGGAATTCTCGGGTGCC1796900.15791165930177667No Hit
AGACCGGGGTTCAATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAG1789920.15729825656265575No Hit
CCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAAC1772170.155738385700278RNA PCR Primer, Index 1 (100% over 24bp)
CCACCGGCCCGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG1749930.15378393341975516RNA PCR Primer, Index 16 (100% over 36bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCT1739980.15290952693633778No Hit
GACCGGGGTTCATTTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAG1655970.14552672405474043No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA1646620.14470504560047387No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACCTGGAATTCTCGG1633740.14357315057470346No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAATGGAATTCTCGGGTG1631020.14333411684255315No Hit
CCTCCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCG1587480.13950781952717706RNA PCR Primer, Index 7 (97% over 36bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGATGGAAT1577870.13866329226027846No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGG1567800.1377783401710309Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAACTGGAATTCTCGGGT1504520.13221729069659358No Hit
ATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1494090.1313007017898556RNA PCR Primer, Index 1 (100% over 32bp)
GACCGGGGTTCAATTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA1487400.13071278426482422RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGC1486820.1306618138366451No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCTGGAATTCTCGGGTGC1479630.1300299562873214No Hit
CCAGTGCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGG1467640.12897627450479132RNA PCR Primer, Index 7 (97% over 36bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCTGGAATTCTCGGGTGCC1444140.12691109336305179No Hit
CGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTAT1415890.12442848199053513RNA PCR Primer, Index 7 (97% over 36bp)
AGACCGGGGTTCAATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAA1415260.12437311755992679No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAATGGAATTCTCGGGTG1413560.12422372147733286No Hit
GGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGG1402130.1232192525220102RNA PCR Primer, Index 7 (97% over 36bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGATGGAATTCTCGGGTGC1400350.1230628260355295No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGTGGAATT1399990.12303118921803903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1386050.12180614134076886No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGGAATTCTCGGGTGCC1374730.1208113391907905No Hit
CGGCGGCGCGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG1374440.12078585397670093RNA PCR Primer, Index 16 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1374360.12077882357281416No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGT1367870.12020848205749972No Hit
ATTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1358890.11941931922120946RNA PCR Primer, Index 1 (100% over 33bp)
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCC1343960.11810727009584049No Hit
TAAACCAGGGGTCGCGAGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1327080.11662385487573142RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGT1308040.11495061875067948RNA PCR Primer, Index 7 (97% over 36bp)
GACCGGGGTTCAATTCCCCGACGGGGAGTGGAATTCTCGGGTGCCAAGGA1302840.11449364249803926RNA PCR Primer, Index 1 (100% over 22bp)
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCTGGAATTCTCGGGTGCCA1302590.11447167248589307No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1286050.1130181364823028No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1270030.11161029810397653No Hit
GACCGGGGTTCATTTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA1266690.11131677874170376RNA PCR Primer, Index 1 (100% over 23bp)
CCACCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTT1250130.10986148513714178RNA PCR Primer, Index 7 (97% over 36bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGTGGAATTCTC1235510.10857667882683406No Hit
CCCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGT1231520.10822603743298125RNA PCR Primer, Index 7 (97% over 36bp)
CAAGTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTA1224660.10762318029969048RNA PCR Primer, Index 7 (97% over 36bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACTGGAATTCTCGGGT1218300.10706426319069204No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1213900.10667759097691953No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA1196850.10517923614855106RNA PCR Primer, Index 1 (100% over 22bp)
ACCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAATGGAATTCTCGGGT1178520.10356839485799424No Hit
AGACCGGGGTTCATTTCCCCGACGGGGAGCTGGAATTCTCGGGTGCCAAG1157850.1017519142537493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)