FastQCFastQC Report
Fri 29 Sept 2023
SRR17657226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17657226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29954433
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG16744225.589897161465216TruSeq Adapter, Index 9 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC5493181.8338454278203162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4376401.4610191419747454No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2607150.8703720080430166No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2146320.7165283348878612No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2081880.6950156592848878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2079090.6940842445590608No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1923650.6421920922355632No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1872870.6252396765447038Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1797750.6001615854321128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1625900.5427911120868154No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1593490.5319713446086594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1482230.49482826131277463Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG1289940.4306340901194825Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1274970.4256364992787545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1110290.3706596616267115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT1083490.36171273881231536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1071210.3576131786570622No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1032550.34470690865689224No Hit
CGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT994600.33203766534322315TruSeq Adapter, Index 9 (100% over 36bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG987670.32972415134681404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC984950.3288161054492335No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA962520.3213280651982296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC938200.31320906658456865No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC909650.3036779230640086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC906140.3025061432476455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC882620.2946542169568024TruSeq Adapter, Index 9 (100% over 37bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC861630.28764690688687045No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT858710.28667209290858553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT831840.2777018012659428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG821720.2743233363822977Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG813180.2714723393362178No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA758720.25329139096039643No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG745110.2487478230684587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG735190.245436126265518Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA709210.23676295258201013No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC703540.23487007749403901Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA660740.22058170822328704No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG612230.204387110248423No Hit
CGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT611290.2040733002691121Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA611240.20405660824893596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA597390.1994329186601529TruSeq Adapter, Index 9 (100% over 35bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC589890.19692911563373608Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA589120.19667205852302397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC576620.19249905347899593No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC558370.18640646611471498No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC553840.1848941690867592No Hit
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG542630.18115181816327486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG539560.18012692812446157No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG517080.17262219585328153No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT491370.16403915907872468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC489020.16325463413044738Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA488120.1629541777672774No Hit
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA485530.16208953112215477TruSeq Adapter, Index 9 (100% over 35bp)
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT470320.15701181858458146Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC468670.15646098191876975No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG467620.15611044949507139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG463050.15458479885097476No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC462000.1542342664272764TruSeq Adapter, Index 9 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC460560.15375353624620436TruSeq Adapter, Index 9 (100% over 50bp)
GGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG450070.15025155041325602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA443290.14798811247737523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC440770.1471468346604992Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT437800.14615532866203812Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCGGTGCGCCGCGACCGGCTCAGATCGGAAGAGCACACGTCTGAACTCC437700.14612194462168587Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC431860.14417231666511596No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA428410.14302056727296422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT422220.14095409517516155No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT403880.1348314621745636No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA399860.1334894237524042Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC392120.13090549902914203No Hit
CGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA391510.13070185638299348TruSeq Adapter, Index 9 (100% over 35bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC387420.1293364491325875Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA385550.1287121675780009No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG381190.1272566234186439TruSeq Adapter, Index 9 (100% over 50bp)
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA374540.125036584735221TruSeq Adapter, Index 9 (100% over 35bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT372900.1244890864734445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC369370.12331062984901099Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT366110.1222223101335285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT364110.121554629326484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG360780.12044294078275491No Hit
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG360530.12035948068187437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG356390.11897738141129227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA356200.11891395173462306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT352480.1176720654335203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG348340.11628996616293821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340390.11363593495493639Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT338830.11311514392544168Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACT329530.11001042817268482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACTCC329020.10984016956688848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA328720.10974001744583181Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG325770.10875518825544118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG323460.10798401692330481No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG323230.10790723363049469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT319370.10661860967289884No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA317420.10596762088603046No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT311330.10393453282857999No Hit
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA304960.1018079694581433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)