FastQCFastQC Report
Fri 29 Sept 2023
SRR17657228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17657228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25487358
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG14109325.535811126441588TruSeq Adapter, Index 9 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4748541.8630962063623857Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3811981.4956356009908913No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2236950.8776704121313791No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1894030.7431252780299943No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1850000.7258500469134541No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1813930.7116979327555253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1689890.66303066798842Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1632980.6407019511398553No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1583330.621221705286205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1423100.5583552441959657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1422150.5579825103880912No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1298380.5094211804926976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG1120820.43975527004407444Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1116990.4382525642712752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA973460.38193837117209245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT946140.3712193315603759No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCT922220.3618342866294733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC911160.357494880403061No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA857880.33659039905195354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT857550.33646092309763925TruSeq Adapter, Index 9 (100% over 36bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTG851480.3340793502410097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC850940.3338674804975863No Hit
CGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAAC835700.3278880455165263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTC805860.3161802804355006No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC786020.3083960291215747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC766910.3008981943126471No Hit
GGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC761630.29882657904361837TruSeq Adapter, Index 9 (100% over 37bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG741820.29105409827099377No Hit
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT741090.2907676817659955Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT725820.28477647624363417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG721190.282959889369467Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG654380.2567468938914736Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG648310.25436532103484405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA642320.25201513628835126No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA640370.25125005110376686No Hit
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC606780.23807096836007874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA567480.22265155925537675No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG549980.21578541016295216No Hit
AACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA538430.21125375176195196TruSeq Adapter, Index 9 (100% over 35bp)
CGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT526310.20649845307622702Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA525460.20616495440602356Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA524340.2057255208641084Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC513200.20135472652755929Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC497230.19508887504150096No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC486500.19087894476940293No Hit
TTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG476420.18692404289216638No Hit
GGCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG467190.1833026396851333Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC466980.18322024589602423No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACG458200.17977540080851062No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA434200.17035896776747123No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT429900.16867185684761835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA425350.16688665808358796TruSeq Adapter, Index 9 (100% over 35bp)
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC423180.1660352555961273Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTC406140.15934958813698932No Hit
CGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT403000.15811760481412002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG401100.15737213719837104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC397130.15581450223283244TruSeq Adapter, Index 9 (100% over 50bp)
GGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG395020.1549866408279744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG394170.15465314215777093No Hit
CCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGAA392750.15409600320284275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC391960.1537860456152419No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC390890.1533662296421622TruSeq Adapter, Index 9 (100% over 50bp)
GGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC382720.1501607188944417Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACGTCTGA378370.1484539904057533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCGGTGCGCCGCGACCGGCTCAGATCGGAAGAGCACACGTCTGAACTCC373400.14650400406350472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACT372130.14600571781508306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT368630.14463248799659814No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT361500.14183502268065604No Hit
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC355720.13956723172327237Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTC353740.1387903759973866No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA349430.13709934156376663Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA347570.13636956800308608TruSeq Adapter, Index 9 (100% over 35bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA342310.1343057997615916No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT330130.1295269599932641Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC330130.1295269599932641Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT324710.12740041553149606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG322870.12667848899834971TruSeq Adapter, Index 9 (100% over 50bp)
GCGGGGCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA322560.1265568600715696TruSeq Adapter, Index 9 (100% over 35bp)
CGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG320010.12555636406095916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG318620.12501099564733228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT317060.12439892749966475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGAACT311200.12209974843214429Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAG310570.12185256706481701No Hit
GCTTTCGCCGAATCCCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA310350.1217662497619408Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA306860.12039694345722299Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG306600.1202949320992784Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACT293580.11518651717451452Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA286850.11254599240925639Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG286130.1122634994180252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAACTCC285550.11203593561953341Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG283810.11135324422405805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTGA282200.110721558507555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACT279890.10981522682735496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA275380.10804572211839296No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG272170.10678627419915396No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT266860.10470288838882397No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC265810.10429091944327851No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGT264950.10395349725930794No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG260350.10214868092644205No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA255920.10041056432761686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)