FastQCFastQC Report
Tue 19 Sept 2023
SRR17658116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17658116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53792019
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length15-89
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT29904405.559263354662334No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT24009774.463444660814832No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC15953922.965852610960745No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC15787332.9348833327858546No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC11686632.1725583492227725No Hit
TCGCTTCTGGCGCCAAGCGTCCG9407621.7488876928006738No Hit
CTCGCTTCTGGCGCCAAGCGTCCG8539491.5875012982873908No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8018911.4907248601321321No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC6045341.1238358612269228No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG5731291.065453594519291No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC5067110.9419817463999631No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC4831350.8981536833558897No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC4676840.869430091478812No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4672660.86865302453139No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC4410270.8198744129682137No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG4260670.7920635959025818No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT4230050.7863713016609397No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3853340.7163404667893205No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC3746780.6965308366655656No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT3687220.6854585621707191No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCC3651140.6787512474666549No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG2729890.5074897820808696No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2685290.49919858929258637No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2668380.4960550002780152No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG2458700.4570752401020679No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG2365000.4396562991993292No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC2173410.40403949143459367No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGG2100600.3905040262571294No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1935990.35990283242575444No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG1919510.3568391809201287No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1881960.34985859147618165No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT1790370.3328319020708258No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCC1767610.32860079113223095No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1675860.31154435753750015No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC1651950.30709946023777246No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1592950.29613129040573843No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC1517640.28213107226928963No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC1473400.2739068039071001No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGG1472460.2737320567945219No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG1459760.27137111176288065No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1423300.2645931546090508No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCG1419280.26384583185100374No Hit
TCGTTTCCCGGTCAGGG1377770.25612907372002525No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG1359880.25280330154553227No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC1345650.25015792770299256No Hit
TGGGGGTGGGGGCCGGGCCGC1297240.2411584514052168No Hit
GTCTAGCGGTTAGGATTCCTGG1248650.23212551289439423No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1230930.22883134392111215No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC1168620.21724784117138268No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1160100.21566396308716354No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1123030.2087726062113415No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1082750.20128450653618338No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1081840.20111533645911303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1049370.19507912502782243No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC1041350.19358819753540019No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1014970.18868412431219583No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC999430.1857952199191482No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT996220.18519847711981216No Hit
GTCTAGCGGTTAGGATTCCTGGT976320.1814990435662956No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC969890.18030369895578746No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA968860.18011222073668587No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT962810.1789875185759434No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCC958060.17810448795387285No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC955630.17765274807773995No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCC951680.17691843840254443No Hit
CGCTTCTGGCGCCAAGCGTCCGTCC932090.17327663421594197No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC919060.17085434179371478No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT889110.16528660134508058No Hit
CCCCGCCCCTTGCCTCTCGGCGCCC884040.16434408234426004No Hit
ATTGATCATCGACACTTCGAACGCA866260.1610387592999623No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCC860380.1599456603404308No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCC825310.15342610583179633No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG798150.14837702968538885No Hit
GTCTAGGGGTATGATTCTCGCTT794680.14773195257831837No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG794360.14767246419956834No Hit
TCGCTTCTGGCGCCAAGCGTCCGTC769870.14311974421335624No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC768470.14285948255632494No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA757430.14080713348944943No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGG738700.1373252043207376No Hit
TCGCTTCTGGCGCCAAGCGTCCGT727350.135215225886948No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG724820.13474489589245572No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG723440.13448835225909628No Hit
TCGGTTGGCCCCGGATAGCCGGGT722990.13440469672647906No Hit
TGGGGGTGGGGGCCGGGCCG721580.13414257605761182No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT714590.1328431267842912No Hit
CTCGGTTGGCCCCGGATAGCCGGGT705010.1310621934454626No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTC698500.129851976740267No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGG696120.12940953192331375No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC693630.1289466379761652No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC687960.12789257826518838No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCC682080.12679947930565685No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT672420.12500367387214079No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGG667070.12400910253991396No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA667060.12400724352807801No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG659170.12254048318952296No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG651660.12114436530073355No Hit
TCGTTTCCCGGTCAGG648700.12059409779729591No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC635430.11812718909100624No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGT618140.11491295762666948No Hit
GTATAGTGGTGAGTATCCCCG615730.11446493577420844No Hit
GTCTAGTGGTTAGGATTCGGCG608370.1130967030629581No Hit
CTCGCTTCTGGCGCCAAGCGTCCGT605150.11249810125178605No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC600660.11166340493744993No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC594370.11049408649264494No Hit
TGGGGAAGGTGGCTCGGGGGG579110.1076572344310036No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC576670.10720363554303475No Hit
CCCCCGCCCCTTGCCTCTCGGC567920.10557700018658901No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGC560450.10418831834514335No Hit
CTCGCCCGTCACGTTGAACGCACGTTC549350.10212481520725221No Hit

[OK]Adapter Content

Adapter graph