FastQCFastQC Report
Fri 29 Sept 2023
SRR17769724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17769724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20974499
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA13393766.385735363690928No Hit
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT11461075.4642878478289285RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG8116253.8695799122544Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC6440453.070609696088569RNA PCR Primer, Index 1 (100% over 24bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG4985342.3768577261368677No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4595902.1911846380693047No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4129051.9686048281773023No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA3718911.7730626128423854RNA PCR Primer, Index 1 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA3587431.710376967764522No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA3345321.5949463202911307No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC2652971.2648550032112804RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2574341.2273666226783295No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG2378871.134172501569644No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG2298701.0959498961095566No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA2213081.055128897238499RNA PCR Primer, Index 1 (100% over 23bp)
ACGAGACTCTGGCATGCTAACTAGTTACGTGGAATTCTCGGGTGCCAAGG2086180.9946268561647169Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA1930600.9204510677466002RNA PCR Primer, Index 1 (100% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGGA1887090.8997068297078276RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1778160.8477723353487491Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT1749110.8339221833141283RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1641580.7826551661615375No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1517840.7236597164966848No Hit
CCTTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT1485950.7084555392717604RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1165360.5556080266803989No Hit
CTAGACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA1141920.5444325511660613RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1139560.5433073753036962No Hit
CGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTC1088640.5190302757648705RNA PCR Primer, Index 1 (100% over 26bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGG1054310.5026627811229246Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG996980.47532958951725135No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG958870.4571599064177886No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG876240.41776444815201547Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG844420.40259364478741544Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA830070.39575200342091604No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG801620.38218791304621863Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA783130.3733724462262483RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG748000.35662353603773805Illumina Small RNA Adapter 2 (100% over 21bp)
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG739600.35261867279881154Illumina Small RNA Adapter 2 (100% over 21bp)
AACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGG718360.34249209003752606Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTGGAATTCTCGGGTGCCAA660030.31468212899864734No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA650040.30991920236092413No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC648070.3089799665775092No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA584080.2784714905466872RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA580810.2769124545001051RNA PCR Primer, Index 1 (100% over 23bp)
AACGAGACTCTGGCATGCTAACTAGTTACGTGGAATTCTCGGGTGCCAAG559740.2668669225424645No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCTGGAATTCTCGGGTGCCAA549620.2620420158784246No Hit
ACTGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA537950.25647811659291603RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA536390.2557343562771154RNA PCR Primer, Index 1 (100% over 22bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG530970.25315026594914136Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG511480.24385802969596557Illumina Small RNA Adapter 2 (100% over 21bp)
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA493330.23520466448328517No Hit
ATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGGA461060.21981931487374262RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA454860.21686334438786836RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG438610.20911584109827844Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA437650.20865814244240113RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC412780.19680088663857953RNA PCR Primer, Index 1 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA411470.1961763186810803RNA PCR Primer, Index 1 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG385900.18398532427401484No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG384450.18329400859586684Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA380860.18158240633065895No Hit
CTAAACCATTCGTAGACGACCTGCTTGGAATTCTCGGGTGCCAAGGAACT355790.16962979664019626RNA PCR Primer, Index 1 (100% over 25bp)
GCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT346580.16523875016037332RNA PCR Primer, Index 1 (100% over 25bp)
TCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGGAA339820.16201578879190393RNA PCR Primer, Index 1 (100% over 23bp)
CCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGGAAC334940.15968915395786093RNA PCR Primer, Index 1 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAA323020.15400606231405098RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA314140.14977234974718584RNA PCR Primer, Index 1 (100% over 22bp)
AGCTAGAAACCCCGAAACCAAACGAGCTGGAATTCTCGGGTGCCAAGGAA294380.14035138574704453RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA293420.13989368709116723RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGCCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAG291830.13913562369237045No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA283180.1350115680951426RNA PCR Primer, Index 1 (100% over 22bp)
ACTAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGGA279920.13345729974289255RNA PCR Primer, Index 1 (100% over 22bp)
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA278420.13274214559308425No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTTGGAATTCTCGGGTGCCA274090.1306777339473043No Hit
TGTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG273270.13028678301207575No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT262740.1252664008804215RNA PCR Primer, Index 1 (100% over 25bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA261270.12456554981360939RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA259860.12389330491278958RNA PCR Primer, Index 5 (100% over 40bp)
ATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGAA258790.12338316161925966RNA PCR Primer, Index 1 (100% over 23bp)
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAA256130.12211495492693294No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG248800.11862023498153638No Hit
GGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAGG243130.11591695229526103Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC225640.10757825490849626No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA221480.10559489406636124RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGGTGGGGGGCCCAAGTCCTTCT220560.10515626618781215No Hit
CACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGGAACT210570.1003933395500889RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)