FastQCFastQC Report
Fri 29 Sept 2023
SRR17769725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17769725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22497927
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC14670286.520725220594768RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA13704966.091654577775099No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG8996753.9989239897524778Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT8979333.991181054147789RNA PCR Primer, Index 1 (100% over 25bp)
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC6464252.873264723456521RNA PCR Primer, Index 1 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA4305921.9139185579186917No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA3579811.5911732667636445No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA3436461.5274562851946314No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAG2625351.1669297353485055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA2484151.1041683973816787No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA2432521.0812196163673213RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCATGGAATTCTCGGGTGCCAAGGAACT2343041.0414470631005248RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG2140730.9515232225617943No Hit
TGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1959170.8708224539976505Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA1790750.7959622235417512RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1753810.7795429330000049No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1707610.7590077076879128No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA1627050.7231999641566976RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG1538920.6840274661749947No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1410220.6268221956627381No Hit
CCTTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT1232850.5479838209093664RNA PCR Primer, Index 1 (100% over 25bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA1184580.5265285108267975RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG1118150.49700134594622875Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACTC1099050.4885116748756452RNA PCR Primer, Index 1 (100% over 26bp)
TAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAG1066520.4740525649318713No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAA1011850.44975254831256234No Hit
TAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT1006490.44737010658804255RNA PCR Primer, Index 1 (100% over 25bp)
CTAGACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA993010.4413784434450338RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG966850.42975070547610894Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG947030.4209410049201422No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGG920830.40929548753536266Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGAA902070.4009569414995435RNA PCR Primer, Index 1 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA868430.3860044527658037No Hit
TAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACT849850.3777459141013303RNA PCR Primer, Index 1 (100% over 25bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCTGGAATTCTCGGGTGCCAAGG789380.35086788218310067Illumina Small RNA Adapter 2 (100% over 21bp)
AGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC706290.31393559059908055RNA PCR Primer, Index 1 (100% over 26bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG685030.3044858310723472No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA645090.286733084341504RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCTGGAATTCTCGGGTGCCAAGGA596660.2652066565955165RNA PCR Primer, Index 1 (100% over 22bp)
ACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGGA586730.26079291660960585RNA PCR Primer, Index 1 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGAA577240.25657475019809606RNA PCR Primer, Index 1 (100% over 23bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGTGGAATTCTCGGGTGCCAAGGA547770.24347576556720094RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA532030.2364795654284059RNA PCR Primer, Index 1 (100% over 22bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG492350.21884238490061775Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCGTCGCCGCGCTCTCCCTCTCCCTGGAATTCTCGGGTGCCAAGGAA490280.21792230013014088RNA PCR Primer, Index 1 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCATGGAATTCTCGGGTGCCAAG463000.2057967385172865No Hit
CGCGCTACACTGACTGGCTCAGCGTGGAATTCTCGGGTGCCAAGGAACTC453280.20147634046461257RNA PCR Primer, Index 1 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG440040.19559135381673165No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGTGGAATTCTCGGGTGCCAAGG439240.19523576549963914Illumina Small RNA Adapter 2 (100% over 21bp)
AACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAAGG438100.19472905214778233Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG425760.18924410235663047Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCA419150.18630605388665364No Hit
CTAAACCATTCGTAGACGACCTGCTTGGAATTCTCGGGTGCCAAGGAACT415380.18463034394235522RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGGTGGGGGGCCCAAGTCCTTCT391650.17408270548659885No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA387840.17238921612644578No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC387330.1721625285742993RNA PCR Primer, Index 1 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC380750.16923781466621346RNA PCR Primer, Index 1 (100% over 24bp)
AAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAACTC364660.16208604463869047RNA PCR Primer, Index 1 (100% over 26bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCTGGAATTCTCGGGTGCCAAGGAAC357260.15879685270558483RNA PCR Primer, Index 1 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAAC333640.14829810764342866RNA PCR Primer, Index 1 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGTGGAATTCTCGGGTGCCAA326600.14516893045301463No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA315380.14018180430579227RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTATGGAATTCTCGGGTGCCAAGGAACT310560.1380393846953099RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAA293260.13034978733818453RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA288520.12824292655941144RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG282590.12560712815896327Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA281620.12517597732448862RNA PCR Primer, Index 1 (100% over 22bp)
AATGCCTAAAGGAAAGATCCAAAAAGATTGGAATTCTCGGGTGCCAAGGA279170.12408698810339282RNA PCR Primer, Index 1 (100% over 22bp)
ACCAGGAGTGGAGCCTGCGGCTTAATTTGTGGAATTCTCGGGTGCCAAGG273930.12175788462643691Illumina Small RNA Adapter 2 (100% over 21bp)
GCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAGGAACT272320.12104226313828824RNA PCR Primer, Index 1 (100% over 25bp)
AACGAGACTCTGGCATGCTAACTAGTTACGTGGAATTCTCGGGTGCCAAG271960.12088224839559662No Hit
AGCCGCCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAAG270630.12029108281843034No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG270440.12020663059312087Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAGG270420.12019774088519355Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAA264910.11774862635121894RNA PCR Primer, Index 1 (100% over 23bp)
ATAATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAA264550.11758861160852731No Hit
CGCGGCGTGGGAAATGTGGCGTACGTGGAATTCTCGGGTGCCAAGGAACT262560.1167040856697597RNA PCR Primer, Index 1 (100% over 25bp)
TAATCCACCTATAACTTCTCTGTTAACCCAACTGGAATTCTCGGGTGCCA255170.11341933859061769No Hit
AGTGCGGTAACGCGACCGATCCCGGAGATGGAATTCTCGGGTGCCAAGGA244650.10874335222085128RNA PCR Primer, Index 1 (100% over 22bp)
AAGCCGCCGTGGCGCAATGAAGGTGAAGGGCTGGAATTCTCGGGTGCCAA242780.10791216452964755No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA241870.10750768281895483RNA PCR Primer, Index 1 (100% over 22bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACTGGAATTCTCGGGTGCCAA240220.10677428191495153No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAATGGAATTCTCGGGTGCCAAGGAA237940.1057608552112379RNA PCR Primer, Index 1 (100% over 23bp)
AATCCACCTATAACTTCTCTGTTAACCCATGGAATTCTCGGGTGCCAAGG235100.10449851668555951Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)