FastQCFastQC Report
Wed 20 Sept 2023
SRR17799053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33291887
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGTTGGATCTCGTAT2969930.8920882135638633TruSeq Adapter, Index 3 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1944220.583992129974489No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGTTGGATCGCGTAT1403390.42154114003811205TruSeq Adapter, Index 3 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT859840.2582731342323732No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT783230.23526152182362028No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT756810.22732565444548097No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT702900.21113252006412253No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483860.14533871270198653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)