FastQCFastQC Report
Sat 23 Sept 2023
SRR17799058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87099367
Total Bases13 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCTCGTAT55286416.347509965256119TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCGCGTAT39094594.4885044916572125TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCTCGTTT3438660.39479735828619744TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCTCGGAT3398140.39014520048119294TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCGCGGAT2416470.2774382964229809TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGTGTTATCGCGTTT2400150.2755645744245191TruSeq Adapter, Index 20 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1536160.17636867556109795No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1059390.12163004583029863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)