FastQCFastQC Report
Tue 19 Sept 2023
SRR17799059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29572208
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTGACATCTCGTAT6809402.3026349605007512TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTGACATCGCGTAT4163061.4077609625902807TruSeq Adapter, Index 4 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634170.21444797087860332No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458850.1551625769709181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)