FastQCFastQC Report
Tue 19 Sept 2023
SRR17799064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35181476
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCGTGAATCTCGTAT5564711.5817159007200265TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCGTGAATCGCGTAT1576950.448233041729119TruSeq Adapter, Index 9 (97% over 37bp)
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC1061560.3017383352534726No Hit
GCTACTACCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGC938120.26665168908774606No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA809830.23018647654237134No Hit
GTCGTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGC639990.18191107161052594No Hit
GCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTC606070.17226963416770802No Hit
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGG605730.17217299240088732No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT563280.16010698357283248No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG559950.15916046273897094No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGGACGCTTGGCTGCCA556940.1583048988621171No Hit
GGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTG554750.15768241218759554No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC529320.1504541765103886No Hit
CCCGGTTCAAACCAATGCGATAAAAATGGTTTCCGCAACAACGAAGTCGT501640.14258639972922113No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA477730.13579020959780083No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA467850.1329819135501876No Hit
CTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATGGTTTCC466170.1325043895258971No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGT441950.12562008484237558No Hit
CTACCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCAT437890.12446606845034017No Hit
GCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTA427670.12156113063590623No Hit
GGCACCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTAC421480.11980168199878821No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA418560.11897169976609281No Hit
GGCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCT415090.1179853852635404No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTACCCCCGGAACCGA414320.11776652008574058No Hit
CCCGACTCCAAGGAGCAAGCGAACCAACAGTGCCGCTATCCAGAACGGGG412690.11730320808598252No Hit
CCTCTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCT398930.11339205893465072No Hit
GCCCTTTTGCTCTACGAGAGGTTTCCGTCCTCTCTGAGCTCGCCTTAGGA393560.11186568749986499No Hit
GTCGGAAACGTCGTCGACTTTCACACCGACGTCACACACACTAGCGTAAC390910.11111245019964483No Hit
CCCGAAGTTACGGATCTAGTTTGCCGACTTCCCTTACCTACATTATTCTA381570.10845764401698213No Hit
CCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGAT379950.10799717442213055No Hit
CGCCCCTATACCCAAGTCTATCGATCGATTTGCACGTCAGAACCGATACG379500.10786926620133845No Hit
CCTCGTTTAAGGGCAATAATTACAATACCCTATCCCGGACATGGAAGAAT370540.1053224714051224No Hit
GGGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGG359140.1020821298117225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)