FastQCFastQC Report
Thu 21 Sept 2023
SRR17799076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47532408
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCTCGTAT4585120.964630279198142TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCGCGTAT3372200.7094528011288636TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCTCGTTT1158900.24381260044725694TruSeq Adapter, Index 16 (97% over 38bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1006990.21185335276933584No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCGCGTTT857680.1804411003120229TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA782360.16459506953655703No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCTCGGAT755640.15897364173092177TruSeq Adapter, Index 16 (97% over 38bp)
CTACGATGTGTATGATTGTGAAGAAGAGGTAGTTCAATAGTCGAACTTGT686990.14453086407909316No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA517310.10883311445109198No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTCAATCGCGGAT503700.10596980485398509TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)