FastQCFastQC Report
Thu 21 Sept 2023
SRR17799077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45392920
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGTAT39498858.701544205572146TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCGCGTAT20318144.476059262105192TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGTTT10741802.3664042762615844TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGGAT6647631.464464061796421TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCGCGTTT5413391.1925626287094993TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGGTT4087180.9004003267469906TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCGCGGAT3047370.6713315644818619TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGTGT2892300.6371698493950158TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCGCGGTT2181330.48054410247236795TruSeq Adapter, Index 25 (97% over 38bp)
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA1628710.35880265028114516No Hit
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGC1379970.3040055585760951No Hit
GCTACTACCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGC1347600.2968744905593207No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCGCGTGT1198430.2640125376380281TruSeq Adapter, Index 25 (97% over 38bp)
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT1068390.2353648983145389No Hit
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGG1048130.23090164721723122No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG1033720.22772714335187075No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC1001100.22054100066706436No Hit
GTCGTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGC950340.2093586400698611No Hit
GCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTC926590.2041265466068277No Hit
CCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGAA906130.19961923577509444No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGGGT843160.18574702839121165TruSeq Adapter, Index 25 (97% over 38bp)
CCCGAAGTTACGGATCTAGTTTGCCGACTTCCCTTACCTACATTATTCTA745920.1643251855135118No Hit
CTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATGGTTTCC735030.1619261329740409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA733110.16150315952355568No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA729310.1606660245694703No Hit
CCCTGAACCAGACGTACCAACTGGAGGCCCAGTTGGTGCTATGCGTTCGA714330.157365950460997No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG703560.1549933337621814No Hit
CCTCGTTTAAGGGCAATAATTACAATACCCTATCCCGGACATGGAAGAAT682410.15033401684668005No Hit
CTACCATTACGATCTGCACTGACGGAAGCTCCAGCCGAGCCTACGCTCAT669980.14759570435213246No Hit
GGCAGAAATCAGTGACAGTCAACGTCCGCAAGGACCATCTGTCACCTCTG661940.14582450302822553No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA659920.14537949971052755No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT655910.14449610203529537No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG642300.14149783710763705No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCTGAGTCCGTACCGGTTCCAGT638090.14057037969797934No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGGACGCTTGGCTGCCA605170.13331814741153467No Hit
GCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTA601340.13247440349728548No Hit
CCCGGTTCAAACCAATGCGATAAAAATGGTTTCCGCAACAACGAAGTCGT595990.13129580560140214No Hit
CTCCGTTACCCGTAACAACCATGGTAGTCCAATAAACTACCATCTAGTTG595240.13113058159730637No Hit
CCTCTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCT588850.1297228730824102No Hit
GCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGA587440.12941225195471012No Hit
GCCCTTTTGCTCTACGAGAGGTTTCCGTCCTCTCTGAGCTCGCCTTAGGA583080.12845174974423323No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA581040.1280023404530927No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGACTAATCTCGTAA575270.12673121711491572TruSeq Adapter, Index 25 (97% over 38bp)
GTCGGAAACGTCGTCGACTTTCACACCGACGTCACACACACTAGCGTAAC573650.12637433326606878No Hit
CCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCAATAAACTACCAT564490.12435639742937885No Hit
CCTGTGGTAACTTTTCTGACACCTCTTACCTGAAACAATCAGGATTAAAA553730.12198598371728454No Hit
CTCGTTTAAGGGCAATAATTACAATACCCTATCCCGGACATGGAAGAATT550380.12124798316565667No Hit
CACCGATGATACAAGGCGTTCGCCAGTACCTTAGGATCGACTGACCCACA531540.11709755618277036No Hit
CCCGACTCCAAGGAGCAAGCGAACCAACAGTGCCGCTATCCAGAACGGGG528250.11637277355147015No Hit
AACCGTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAG527190.11613925695901477No Hit
GGCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCT519340.11440991238281212No Hit
CTCCGAATCGCGAGATGGCATCGTTTACGGTCAGAACTAGGGCGGTATCT517720.11405302853396522No Hit
CCCACGTCGGCCAATTCGAGACTGACCTTTACTTTCATTTCGCCGCAAGG517450.11399354789249072No Hit
CTGGAAACTTACACCTATACGCCACATTTCGAAGCAGCCAAAGACTGATC493440.10870417677470408No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTACCCCCGGAACCGA487580.10741322655603561No Hit
ACCGAATATAACTGCCTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAG485660.10699025310555038No Hit
CCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGA485290.10690874259686313No Hit
CTTCCTTGGACGTGGTAGCCGTTTCTAAGGCTCCCTCTCCGGAGTCGAAC482040.10619277191244803No Hit
GGCACCTTAACTCCACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTAC478420.10539529071934566No Hit
CTGAAAGTCAAGATCAAGCAAGCTTTTGCCCTTTTGCTCTACGAGAGGTT472650.10412416738116868No Hit
GTCCGTTCCTTAACTAGAGAAGAAAACTCTAGCTCGGTCTTTGAGCGACT472570.10410654348739848No Hit
CCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCGATCAGGAT461570.1016832580939935No Hit
GTTCGAATGCGTTACCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATC459340.10119199205514869No Hit
GTTCCAACCACTGGAGCCAATCCTTTTCCCGAAGTTACGGATCTAGTTTG455180.10027554957909735No Hit
ACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATGGTTTCCGCAACA454350.10009270168123134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)