FastQCFastQC Report
Tue 19 Sept 2023
SRR17799078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17799078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35251104
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCTCGTAT11316553.2102682514567484TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCGCGTAT9185402.6057056255599824TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCTCGTTT1833080.5200064088772935TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCGCGTTT1490060.42269881817034727TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCTCGGAT1350590.38313409985684416TruSeq Adapter, Index 7 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1241630.35222442962353745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1089950.30919599000360387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCGCGGAT1019250.28913988055523027TruSeq Adapter, Index 7 (97% over 37bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569470.16154671354406377No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCTCGTGT548260.15552988070955168TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCTCGGTT507960.14409761464492005TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCGCGGTT468290.13284406638725413TruSeq Adapter, Index 7 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT465870.1321575630652589No Hit
CTCGGATTCGAGTCCACGGCGGCACTCAGAGACTGGCTCGATGGAGAAGC438310.12433936820815598No Hit
CTACGATGTGTATGATTGTGAAGAAGAGGTAGTTCAATAGTCGAACTTGT430890.12223446959278213No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418150.1186203983852534No Hit
CTCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAA413880.11740908880470807No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAGCTCATCGCGTGT374710.10629738007638001TruSeq Adapter, Index 7 (97% over 37bp)
GGCGTGGCATGCAAGTGAAACGGATCTTCTTGTTGGAAGTCTTCTCGGAT360540.10227764781494504No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)