FastQCFastQC Report
Sun 1 Oct 2023
SRR17920633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17920633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288830667
Total Bases14.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA113955313.9454020303183386No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA99538513.446258357323255No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC76675272.654678978392554No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA55827611.9328837404928336No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG50280301.7408227638099107No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA37044651.2825732940609107No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA25238160.8738047196352594No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC23765920.8228322929434637RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA20223000.7001680330572376No Hit
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAG18997720.6577459449622778RNA PCR Primer, Index 31 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA14628530.5064742657676305No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA13281450.4598351739429387No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA12875030.4457639534516603No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC10540770.3649463579987509No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA9554510.33079970694386135No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA8980680.3109323567777517No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA8853120.30651592824109636No Hit
CGCGCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTA8461380.29295296402857385RNA PCR Primer, Index 38 (97% over 40bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG7769150.26898632616459667No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGC7719680.267273557900969No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA7076460.24500376201395538No Hit
CGCCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGA6626790.22943512435263672RNA PCR Primer, Index 38 (97% over 40bp)
GACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA6533050.2261896241094094RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGATC6504480.22520046321812498RNA PCR Primer, Index 38 (97% over 40bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG6398730.2215391484035177No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGATCTCGTAT5812030.20122620843443886RNA PCR Primer, Index 38 (97% over 40bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC5360120.18558001668153887No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG5249430.18174766739710504No Hit
ACGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGA5154580.17846373633171023RNA PCR Primer, Index 38 (97% over 40bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG4961130.17176604034224663Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGA4599000.15922824427781415RNA PCR Primer, Index 38 (97% over 40bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA4514530.15630369333322905No Hit
CCAGTGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAG4475310.1549458042833104RNA PCR Primer, Index 38 (97% over 40bp)
AGAAGTCAGCAACTTGATTCCAGCAGTAGTTGGAATTCTCGGGTGCCAAG4409870.15268011689354302No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC4398060.15227122679462565No Hit
TCTGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTA4227260.1463577273115531RNA PCR Primer, Index 38 (97% over 40bp)
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4178330.1446636551235745No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG4139960.14333519508162199Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAG3974230.13759723097547671RNA PCR Primer, Index 38 (97% over 40bp)
CCAGTGCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCT3782740.13096739481614672RNA PCR Primer, Index 38 (97% over 39bp)
CGCGGGCCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCT3744100.12962958673636965RNA PCR Primer, Index 38 (97% over 39bp)
CGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTA3730510.129159068832535RNA PCR Primer, Index 38 (97% over 40bp)
CCCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTA3541610.12261890459159587RNA PCR Primer, Index 38 (97% over 40bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA3339890.11563488166580317No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG3193050.1105509339837518No Hit
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGA3165970.10961336041231384RNA PCR Primer, Index 38 (97% over 40bp)
CCGCGAGGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAG3164840.10957423714290007RNA PCR Primer, Index 31 (97% over 37bp)
CGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGAT3147310.10896730713155192RNA PCR Primer, Index 38 (97% over 40bp)
CGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGATC3109160.10764646400930826RNA PCR Primer, Index 38 (97% over 40bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG3060860.10597420390958694No Hit
CCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAGCTAGAT2958320.10242402687800463RNA PCR Primer, Index 38 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)