FastQCFastQC Report
Sat 30 Sept 2023
SRR17920634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17920634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126028808
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA59091974.688766873046994No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA29564912.3458850773229565No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA26489342.101848015574344No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA14859101.179024084715615No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA14103581.119075886205319No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAA12096520.9598218210553893No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG11613410.9214885218941371No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA10627550.8432635497115865No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8906560.7067082630821995RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC7744890.6145333057502218No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA7052760.5596149096324072No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT6608040.5243277394165308No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC6086400.4829372027385993No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5608140.4449887362260857RNA PCR Primer, Index 1 (100% over 31bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC5592480.4437461631788186No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC5369770.42607480664262093RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGC5185120.41142339456229726RNA PCR Primer, Index 7 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA5056080.4011844656977157No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA4876280.38691788626613055No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG4784340.37962272879705405No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA4750780.3769598455616592No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4739630.37607512720425No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGATGGAATTCTCGGGTGCC4676720.3710834113419529No Hit
CCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG4673210.3708049035899792RNA PCR Primer, Index 1 (100% over 29bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGG4268550.33869637170574524No Hit
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG4205080.33366022155823294RNA PCR Primer, Index 16 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA4110480.3261540012343844No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGGGT4074220.3232768812667021No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCTGGAATTCTCGGGTGCCAA3676560.2917237779476578No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGTGGAATTCTCGGGTGCCAAG3613950.28675586616672594No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGATGGAATTCTCGGGTGC3340120.2650282941658863No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA3332220.2644014533566008No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA3119790.24754578334185307No Hit
CGCGCGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGT3102110.24614292948005984RNA PCR Primer, Index 7 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA3049150.24194071564971084No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCTCG3021120.23971662098081575No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGTGGAATTCTCGGGTGCCAAG2971920.23581275163691148No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC2967800.2354858422528284No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC2782340.22077015915281845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC2779530.22054719425736377No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC2770600.21983862610205754No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG2739040.21733443674243114No Hit
CCCGCGAGGGGGGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2710780.2150920922778227RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2513780.19946074551462867No Hit
AGCAGAGTCGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2410600.19127372846373347No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA2404270.19077146234692627No Hit
CACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC2398290.19029696765837856RNA PCR Primer, Index 16 (100% over 35bp)
GACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGCCAAGGAA2286950.181462479594348RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGG2254620.1788971930925507No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2052580.16286593776242017Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGT2001600.1588208308690819No Hit
CGCCGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTA1993670.15819160965165996RNA PCR Primer, Index 7 (97% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG1969640.15628490273430184No Hit
CGCGGGCCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGG1960940.155594584374709RNA PCR Primer, Index 7 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1896190.15045687014670486No Hit
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCTGGAATTCTCGG1778040.14108202943568268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGTGCCAAG1745320.13848579762811053No Hit
CCCCGGGGAGCCCGGCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1701120.13497866297362743RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG1688420.13397095686249766No Hit
CGCCCGGGGCACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTC1679760.1332838123804202RNA PCR Primer, Index 1 (100% over 26bp)
CACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCTC1639260.13007026139610875No Hit
CGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGCGGTTATC1623260.128800710389961RNA PCR Primer, Index 7 (97% over 36bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTG1586190.12585931940259246No Hit
GTGGGGGGGCCGGGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1525460.12104057986488295RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1479300.11737792521214674No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1428340.11333440525756618No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGGAATTCTCGGGTGCCA1411630.11200851792552065No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAAGGA1368660.10859898000463514RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGC1367370.10849662245476448RNA PCR Primer, Index 7 (97% over 36bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG1366520.10842917755756287No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGATGGAATTCTCGGGTGCC1329840.10551873187596918No Hit
CCGCGAGGGGGGCCCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1326120.10522356126703983RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG1298880.1030621506790733No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCTGGAATTCTCGGGTGCCAA1280040.10156725436933436No Hit
AGCAGAGTAGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1262990.10021438907840817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)