FastQCFastQC Report
Fri 30 Jun 2023
SRR1795425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1795425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66704290
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA30177894.524130307061211No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA25975753.894164828079273No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA16390982.4572602451806325No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT15162672.2731176660451675No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA14541102.179934753821681No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT14520372.1768270076782166No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA14421822.162052845476655No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC11327391.6981501489634325No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA10030201.5036813974033754No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT7576721.13586697347352No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA7214761.0816035970100275No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6790681.0180274761938102No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT6301880.9447488309972267No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA5963150.8939679891653146No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG5910240.8860359656028121No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT5903670.8850510214560413No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5361640.8037923797704766No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG4337010.650184568338858No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT4313210.6466165819319867No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT4291510.6433634178551335No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA3851520.5774021431005412No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3505450.5255209222675183No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA3096990.46428647992505434No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGAT3010250.4512828185413562No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG2292990.3437545021467135No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGA2268220.3400410978064529No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGAT2238690.3356140961848181No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2095050.3140802488115832No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2072190.3106531828762438No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1835060.2751037452013956No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1739920.26084079449762526No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1706140.255776652446192No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1706120.25577365413828707No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1665360.24966310262803187No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1664970.24960463562388566No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1582720.23727409436484517No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1560750.23398045313127538No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATC1525190.228649461676303No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATTAATA1511280.22656413852842147No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1464290.2195196141057794No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCG1386210.20781422004491767No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1369440.20530013886663062No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1361400.2040948190888472No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATTAATA1342620.20127940796611432No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1329880.19936948583067146No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1293830.19396503583202818No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1293710.19394704598459858No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC1281560.19212557393235127No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1199130.1797680479021664No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1191300.17859421035738482No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1184400.17755979413018264No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC1178410.17666180091265496No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACTATCAATA1156770.17341763175951652No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1107730.1660657807766187No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATC1105200.1656864948266446No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCATCATCAATA1102380.16526373341204892No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1084960.16265220722685153No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACTATCAATA1060920.15904824112512103No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1046120.15682949327547No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC981000.14706700273700538No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT972250.1457552430285968No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATTAATA943860.14149914495754323No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATTAAT927260.1390105493964481No Hit
ATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCAATAGATCGG920520.1380001196324854No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCATCATCAATA913410.1369342211722814No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA903210.13540508414076516No Hit
ATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGATCGGA896870.13445462053490112No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC894500.13409932104816646No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGTACCATCAATA881050.13208295898209846No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA879950.1319180520473271No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA823030.12338486774988536No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC795340.11923371045550445No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA782790.11735227224515844No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGTACCATCAATA780300.11697898291099418No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA767520.11506306415974145No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG760550.114018153854872No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA751360.11264043137255489No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT730620.1095311860751385No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGTACCATCAATA725930.10882808287143152No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT725250.10872614040266376No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCG724450.1086062080864664No Hit
TTGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCAATAGATCGG723360.10844280030564751No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGG717120.10750732823930816No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT708990.10628851607595255No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACTATCAATA700930.10508019799026419No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAG694720.10414922338578224No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA672090.10075663799134957No Hit
TGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA668010.10014498317874308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)