FastQCFastQC Report
Fri 30 Jun 2023
SRR1795429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1795429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35387242
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8795672.485548322754285No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA8568502.421352870619304No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA8180602.3117370944025533No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG7322042.0691185823410594No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT6003011.6963769032918699No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA4600131.2999402440009311No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4438061.2541412523756443No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3666981.0362435139760255No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3660441.0343953902934848No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2926450.8269788303931682No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA2812970.7949107760361771No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA2226910.6292974174138803No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT2198560.6212860555790135No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2030570.5738141446569924No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1759810.49730069384893005No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1665940.47077418466237064No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1621940.45834032502448196No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT1471600.4158560873435686No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1464630.41388645094183946No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGAT1461220.412922826819903No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG1369660.38704909526433284No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA1297120.36655018212495905No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1210500.3420724339014609No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCGGA1147530.32427788523332784No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA1114090.31482815190853247No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1054490.29798592385357414No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA1012770.2861963642151033No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG1006080.2843058523747061No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG980880.2771846418548244No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT911400.25755044713572195No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC899390.2541565686300164No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA896600.25336814889388665No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA895070.25293578968375097No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG843890.2384729502231341No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT829630.23444324934958197No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT829070.23428500022691792No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC823660.2327562006668957No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT761120.21508316471795116No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA747970.21136713621253672No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA708950.2003405634154818No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGAT694070.19613565815612305No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG686260.1939286480703978No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATAGA675190.19080040202059262No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG643540.18185650071288403No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA637260.18008184983729447No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATC624960.17660602089306648No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT609240.1721637419497117No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC598470.1691202722156194No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC562710.15901493538264438No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA555520.15698312968272576No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA544660.1539142270539196No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGA538960.15230347705537492No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATC495280.13996004548757995No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT468090.1322764854068028No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA460690.1301853362858852No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG457900.1293969165497554No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAG457810.12937148365504156No Hit
ATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCAATAGATCGG454390.12840503365591474No Hit
TAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATAGATCGG450030.1271729512008876No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA444510.12561306699177063No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCGG430230.12157771436383767No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG424860.12006021831257718No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTGTAGGCACCATCAATAGATC424290.1198991433127227No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT423850.1197748047163438No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG423830.11976915296196294No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTCTGTAGGCACCATCAATAG418470.11825448278789288No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC417560.11799732796356381No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC404330.11425869244062592No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT395140.11166171130262144No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA378820.10704987972784089No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA374010.10569063279924443No Hit
ACAGTGAAACTGCGAATGGCTCATTCTGTAGGCACCATCAATAGATCGGA370940.10482308850178264No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG366550.1035825284151842No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC362070.10231653543387191No Hit
AAGAGGGACGGCCGGGGGCATTCGTCTGTAGGCACCATCAATAGATCGGA361250.10208481350425669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)