FastQCFastQC Report
Fri 30 Jun 2023
SRR1795431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1795431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31993886
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC14850304.6416055867674215No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA5036711.57427265947No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT4625091.4456168281652313No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4439301.3875463580760399No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT3761011.1755402266545552No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3525201.1018355194489347No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3040790.9504284662388307No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT2997080.9367664809457656No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2562650.8009811624633532No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1918830.5997489645365368No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA1867240.583624008662155No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1817870.5681929353627128No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1628810.5091003949942186No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1463290.45736551039783035No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1369300.42798802246154155No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1358450.42459674951645443No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1337220.4179611066939477No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA1324680.41404160782469496No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1305530.4080560892165459No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1204820.3765781999723322No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1197410.3742621324586829No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1155010.3610096003967758No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1132860.3540864026333031No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1113630.3480758792476788No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1112150.3476132908643858No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA1024500.3202174315430142No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1019670.31870776810294316No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA984600.3077462987772101No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA982980.3072399520333354No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT857340.2679699490083824No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCGGA856800.26780116676042415No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA778340.24327773125152724No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGG753890.2356356461356398No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCACCATCAATAG736660.23025024218689782No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT695630.2174259169392552No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA638160.1994631099204392No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT602400.1882859743889817No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC568220.17760268321266132No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTCTGTAGGCACCATCAATAGA543800.1699699748883271No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCG524700.16400008426610008No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA520770.16277172457262615No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC505990.15815209193406515No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT482440.15079131056477477No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG472090.14755631747890832No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG468600.14646548406154852No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT455580.14239595652744402No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG453400.14171457634124218No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC433490.1354915123470778No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC431550.13488514649330188No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC427350.13357239567584883No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA424520.1326878516726602No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAT423190.1322721472471334No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG407660.12741809481974148No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG405160.1266366955236385No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCTGTAGGCACCATCAATAGAT400330.12512703208356749No Hit
ACAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC395370.12357673588009908No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAAT377490.11798816811437035No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA363280.11354669451532085No Hit
TAACGGCGAGTGAACAGGGAAGAGCCCCTGTAGGCACCATCAATAGATCG357990.11189325360476687No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGCTGTAGGCACCATCAATAGA357330.11168696419059568No Hit
TATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCGG353840.11059613077323587No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT351980.11001476969693522No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA350710.10961781885451488No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA343300.10730175134086556No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC342580.10707670834358789No Hit
TAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCAT339740.10618903874321486No Hit
ATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCAATAGATCGG337920.10562018005565188No Hit
TAGTGACGAAAAATAACAATACAGGACTGTAGGCACCATCAATAGATCGG336190.10507945174274859No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG333520.10424491729451059No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG327350.10231642383172834No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC326120.10193197537804567No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT325950.10187884022591068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)