FastQCFastQC Report
Tue 19 Sept 2023
SRR18017736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36279258
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN29575258.152109946680829No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN16178384.459402118973878No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN15768994.34655802497394No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15710714.330493749348457No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN15673314.320184828476922No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN13726523.7835724203620704No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN12623423.479514382570889No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN9295472.562199590741354No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN8839752.436585114282106No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8062092.2222312264490083No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3310750.9125737907870111No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2539990.7001218161628333No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN2465280.6795287819833581No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN2302400.634632604669037No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2234600.6159442400944363No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN2198570.6060129454687303No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN2116050.5832671660484346No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1998260.5507995780950096No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1940490.5348758786632295No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1835110.5058289780899048No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN1832000.5049717389479135No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1687090.4650288051646481No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN1666800.45943607777204265No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1638540.4516465028033374No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1408510.38824112665148774No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1289400.3554096944320085No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1251810.34504840203732945No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN1216980.33544787492621814No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1131020.3117538952974176No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1076290.2966681402359442No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1031540.2843332683375167No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN973230.26826072352416913No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN966140.2663064387921054No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN963180.26549054558943846No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN883910.24364059485450337No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN873650.24081253260471863No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN830900.22902893989728235No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN828600.22839496882764249No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN784980.21637156967212506No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN772830.21302254858685368No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN772100.21282133168214187No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN734850.2025537567499313No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN733800.20226433517466094No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN696840.1920766957251441No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN680740.18763889823766516No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNN665860.18353738105669085No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN659290.18172642891428484No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN649200.17894522539573438No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN643820.17746228437196815No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAANNNNNNN569730.15704014674170017No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGNNNN563960.15544970627569063No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN556660.15343753722857287No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN544700.15014088766644565No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN532010.14664302119960668No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN517800.1427261825476144No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN516440.1423513126977404No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN507250.13981818481513594No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN496250.13678614926468452No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN488940.13477122382161177No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN488590.13467474996318834No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN487330.1343274440728639No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN469790.1294927255678713No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA440340.1213751394805263No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN438360.12082937308144505No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN432760.11928579134666978No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN425870.1173866345337052No Hit
CAACTTCTTAGAGGGACAAGTGGCGTNNNNNNNNN414470.11424434314505551No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAANNNNNN404270.11143281927100052No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN400570.11041295276766686No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN399420.11009596723284693No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN398490.10983962240903604No Hit
CCCCATTCGTGATGGGGATCGGGGATTNNNNNNNN386000.10639688386129616No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGANNNNNNNN384280.10592278375704375No Hit
CGACTTCATAATTTGTGGTAGTGGGGNNNNNNNNN376790.10385824318678183No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN374730.10329042562006092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)