FastQCFastQC Report
Tue 19 Sept 2023
SRR18017737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39751977
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN37351929.396242103883285No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN36429509.164198298866998No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN30326247.62886333930008No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN24365136.129287607506917No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN13662543.4369460417025293No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11614972.921859710273026No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN6110471.5371487058366935No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN5122481.288610123717872No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN4453821.1204021374836275No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN4328481.08887163020848No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN3897520.9804594121193018No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3831760.9639168386518235No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN3531930.8884916591695553No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN3486700.877113608714354No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN3182580.8006092376235778No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN3093620.7782304764364298No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN3061490.770147859564318No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN2763780.6952559868909162No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN2410770.6064528564201976No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN1983890.4990670023782716No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN1541130.3876863784661578No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN1505260.37866292788406475No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1426180.3587695776740865No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNN1185540.2982342236714416No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTNNNNNN1131910.28474307076601496No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN1002540.2521987774343903No Hit
TCGACAGCATAATTTGTGGTAGTGGGGNNNNNNNN976460.24563809744607168No Hit
TCGACAGCATAATTTGTGGTAGTGGGGGNNNNNNN935510.2353367230012233No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN910320.2289999312487024No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNN900460.22651955146784272No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN854830.21504087708644026No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN838880.2110284980291672No Hit
CGACTTCATAATTTGTGGTAGTGGGGGNNNNNNNN830020.20879967806381053No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN795930.20022400395331283No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN762120.19171876659115594No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN757720.1906119034029427No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN752200.18922329322136605No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNN749280.1884887385600973No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN742850.1868712089464129No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN716690.18029040417285408No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN714790.17981244052339837No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN709690.178529485464333No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN700150.17612960482443427No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNN690410.1736794122214349No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN672740.1692343502814967No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN658720.16570748166814447No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNNN657720.16545592185264146No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN643520.16188377247249866No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN635540.15987632514478461No Hit
TGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN625640.15738588297130482No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN620910.15619600504397554No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN598600.15058370556010334No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN592650.14908692465786041No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN571830.1438494492990877No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNNN566110.14241052715441047No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNN560200.14092380864478765No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNN547750.13779188894177516No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN547070.1376208282672331No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGNNNNNN529060.13309023599002384No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN518010.13031050002871555No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN511480.12866781443348088No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN505240.12709808118474208No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN486200.12230838229756473No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNNN473400.11908841665912616No Hit
GCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNN468720.11791111672257205No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN467420.11758408896241815No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN456960.11495277329225664No Hit
TCGACTGCATAATTTGTGGTAGTGNNNNNNNNNNN452740.11389119087083394No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNNN450000.11320191697635566No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN439100.11045991498737284No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN430910.10839964009840317No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNN429180.10796444161758295No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN429020.10792419204710246No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGNNN427170.1074588063884219No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTNNNNNNN426750.10735315126591063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)