FastQCFastQC Report
Tue 19 Sept 2023
SRR18017738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37661865
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN26589967.06018143286319No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN24873586.604447230640331No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN18976225.038576820345992No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN11719923.111879881678722No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN8716742.3144738052669456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8076242.1444078778361084No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN7674522.0377429529844044No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN5949801.5797943091772009No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5137361.3640747743108315No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN5124541.360670800556478No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN4717231.252521615698001No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN4276881.1355996310857148No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN3898251.0350655762798788No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN3248900.8626497917721281No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN3195390.8484417858754472No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN2312530.6140242922117638No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2270260.6028007375630495No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN1968010.522547144173556No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN1965790.5219576885000252No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1940170.5151550514027917No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN1865320.49528083646415283No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNN1451610.3854323199342359No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1442200.38293377133607165No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNN1414390.37554964418251724No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1350970.35871032940084085No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1314730.3490878638113115No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1289810.3424710911156418No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1230520.32672837630319157No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1203800.3196336665749293No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNN1187180.31522071464065843No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN1137810.3021119639189403No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNN1133490.30096491504071826No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNN1098080.2915628315273288No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1055610.28028617276388196No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1022560.27151071780433605No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN988300.26241398295065843No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN982060.2607571345710044No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN977090.2594374973199017No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGNNN969010.25729209108470863No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTNNNNNN925860.24583487833117132No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN902710.2396880770508842No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN866870.23017181969081987No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN866480.23006826666709151No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNNN808190.2145910724283038No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN788490.20936031712715236No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNNN709930.18850102086022558No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNN696680.18498287325919735No Hit
TCGACAGCATAATTTGTGGTAGTGGGGNNNNNNNN688960.18293305443052274No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN688040.18268877550275325No Hit
TCGACAGCATAATTTGTGGTAGTGGGGGNNNNNNN681590.18097616780263007No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN679500.1804212298036754No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN669860.17786161147356883No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNN669160.17767574707200506No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNN668260.1774367785557088No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGNNN644040.17100587026160283No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNN618640.16426164769057508No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGANN609370.1618002719727236No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN605890.16087626037637806No Hit
CCCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN601460.15970000423505315No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN572160.15192025142674162No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN563360.14958367037851153No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGNNNNNN561330.14904466361397664No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNNN558930.1484074142371866No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN558620.14832510285935122No Hit
CGACTTCATAATTTGTGGTAGTGGGGGNNNNNNNN553160.14687536052715391No Hit
TCGACTGCATAATTTGTGGTAGTGNNNNNNNNNNN547520.14537782449169737No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN524620.1392974033548259No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGNNNNN521350.13842915107894949No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN503510.1336922640448103No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGNNNN502120.13332319044741942No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN501320.13311077398848942No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNN486920.12928727772774928No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN476070.12640637950351105No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN474420.1259682705569679No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN470680.12497522361147012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN470220.12485308414758536No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGNNNNN464130.12323606385398068No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN461170.12245012295593964No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN453330.12036844165842558No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCNNNNN447720.11887887124017889No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTNNNNNNN446830.11864255792961927No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA443710.11781413373979223No Hit
TCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNN438130.11633252893875543No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGNNNNN431800.11465178370747174No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNN424640.11275065640004817No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN418360.1110831871974476No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN413080.10968123856850956No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNN412360.10949006375547254No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAANNNNNN409910.10883953834999939No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGNNNNNNN409890.10883422793852614No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNNN408590.10848905119276488No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN405990.10779869770124237No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN404550.10741634807516834No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNN403510.10714020667855932No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN395160.10492310988847738No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGANN390680.10373357771846932No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCNNNNNN384850.10218559277401691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)