FastQCFastQC Report
Tue 19 Sept 2023
SRR18017739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22918936
Total Bases838.4 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN258780611.29112625472666No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN19791808.635566677266345No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN13658515.959486950005009No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN5659832.4694994566938013No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN4371091.907195866335156No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3869881.688507703848032No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3666711.599860482179452No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN3497461.5260132494806915No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3090391.348400292229971No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3037651.3253887527763069No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2920071.2740861966716082No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN2901431.26595318386508No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN2851561.2441938840441809No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1662330.7253085396285411No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1420140.6196360947995142No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1370650.5980425967418382No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1322920.5772170226401435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1249220.5450602069834306No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1193310.5206655317681415No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1141750.49816885042132847No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1120350.48883159322928427No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1049400.4578746587537921No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN978860.42709661565440915No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN973620.4248102966036469No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN927070.40449958060880314No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN783020.34164762273431887No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA748620.32663819995832266No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN717760.31317335150287956No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN699700.3052934045454815No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN689400.3007993041212733No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN683570.2982555560170856No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN668780.2918023768642663No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN649010.2831763219723638No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN633010.27619519509980744No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN597780.26082362636729733No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN576140.2513816522721648No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN548240.2392083122881446No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN534200.2330823734574764No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN512740.22371893703966014No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN479430.2091851035318568No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN472020.2059519691490041No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN462170.2016542129180866No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN451390.19695067868770172No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN439820.1919024513179844No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN437450.19086837189998698No Hit
CCCCATTCGTGATGGGGATCGGGGATTNNNNNNNN436550.19047568351340569No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN425600.1856979748099999No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN414430.18082427561209646No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN411810.1796811160867154No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN410530.17912262593691086No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN408390.17818890021770645No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN379190.16544834367529104No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN378220.16502511285864233No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN376010.16406084470937046No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN350900.15310483872375227No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN350290.15283868326173605No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN341020.14879399287994868No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN338230.14757665888154667No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN330310.14412100107963127No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN329950.14396392572499875No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN307490.13416416887764773No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN304430.13282902836327132No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN303860.1325803257184365No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN301040.13134990210714842No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN290480.12674235837126122No Hit
GCCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNN263240.11485698987073395No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN251240.10962114471631666No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN249180.10872232463147503No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGNNNN245680.10719520312810332No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN233860.10203789565100231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)