FastQCFastQC Report
Tue 19 Sept 2023
SRR18017740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39185167
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN839720321.429545011253875No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN500958712.784396197673473No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN9537172.433872490577876No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN6853981.7491261425528697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6083861.5525925919876773No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5381051.3732364596021756No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN3635510.9277770846300082No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN2233950.5701009261999572No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN2233800.5700626464090354No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2149350.5485111241200018No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN2014970.5142175354260963No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN2008700.5126174401655607No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN1996590.509526985045132No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1878280.47933443795199343No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN1853840.47309738401778406No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1797740.4587807422129909No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1680070.4287515222277858No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN1619120.41319716718318433No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1576430.40230273868680977No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1443720.3684353316651681No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN1346300.3435738834544204No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN1313170.3351191536328019No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1263260.3223821912000528No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1243330.31729608297956213No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1163590.2969465461254765No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1156620.2951678118406386No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1044500.266554944119544No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN1025560.2617214825191379No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1017830.259748797293629No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1006290.2568038053787036No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN992890.2533841440563466No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN982330.2506892467754444No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN981270.2504187362529296No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN891130.2274151338949251No Hit
CCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNNN885180.2258967021883561No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN870510.22215293863619362No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN866060.22101730483884374No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN844680.2155611586394413No Hit
CCGAATCCCGGGGCCGAGGGAGCGANNNNNNNNNN827130.21108242310157818No Hit
GCCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNN825710.21072004108085082No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN805290.2055088855433486No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN783060.199835820528722No Hit
CCCCATTCGTGATGGGGATCGGGGATTNNNNNNNN769870.19646975091365568No Hit
CGAATCCCGGGGCCGAGGGAGCGANNNNNNNNNNN761530.19434139453839766No Hit
CGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNNNN740450.18896180792083903No Hit
GCCGAATCCCGGGGCCGAGGGAGCGANNNNNNNNN740010.18884952053413476No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN734030.1873234328693814No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN732670.1869763627650228No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN731260.18661653273035686No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN702240.17921066917999864No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN675850.17247597796380454No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN635010.16205366688880005No Hit
GAATCCCGGGGCCGAGGGAGCGNNNNNNNNNNNNN610330.15575536528911565No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN608110.15518882438347142No Hit
TCGCCGAATCCCGGGGCCGAGGGAGCGANNNNNNN598460.1527261578341621No Hit
GAATCCCGGGGCCGAGGGAGCGANNNNNNNNNNNN590200.15061821734739578No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN562580.143569631845642No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN546460.13945583031456776No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN543020.13857794710942536No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN527120.13452028927170323No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA521320.1330401373560562No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGNNNN519890.13267520334926733No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN498380.1271858813310659No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN496770.12677501157517077No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN495860.12654278084357787No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN482140.12304145596725413No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAANNNNNNN479940.12248001903373285No Hit
CCATTCGTGATGGGGATCGGGGATTNNNNNNNNNN479710.12242132335431924No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN466610.11907822161380606No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN465450.11878219123067665No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN437620.1116800140216322No Hit
GGCGGGGCGCGGGACATGTGGCGTACGNNNNNNNN417130.10645099458169976No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGNNNNNNN416200.10621365987798392No Hit
CGCCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNN403990.10309768489694073No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN397170.10135723040302469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)