FastQCFastQC Report
Tue 19 Sept 2023
SRR18017741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29999790
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN379110612.637108459759219No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN28953879.651357559502918No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN22968737.656296927411825No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN6077802.0259475149659383No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3852301.2841089887629213No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN3658351.2194585362097534No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3330231.1100844372577274No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3291041.0970210124804207No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN3252941.0843209235797984No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN2418480.806165643159502No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2323960.774658755944625No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2017670.6725613745962888No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1783090.5943674939057907No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA1630030.5433471367632906No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1436970.4789933529534707No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1403040.46768327378291646No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN1371330.45711319979239856No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1352270.4507598219854206No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1273270.42442630431746353No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1203460.40115614142632333No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1144430.3814793370220258No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN1087820.3626092049311012No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1073690.3578991719608704No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1057130.3523791333205999No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1042790.3475990998603657No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1021950.34065238456669195No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1002070.3340256715130339No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN945320.31510887242877367No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTN938920.31297552416200247No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN877420.292475380660998No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN855450.28515199606397246No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN854950.28498532823063094No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN835060.27835528182030606No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN803970.26799187594313156No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN798090.26603186222303554No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN790660.2635551782195809No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN768310.2561051260692158No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN767850.2559517916625417No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN737760.24592172145205016No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN730150.24338503702859252No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN713620.23787499845832255No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN668890.22296489408759196No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN667650.22255155786090502No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN642820.21427483325716615No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN557810.1859379682324443No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN549160.18305461471563633No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN519760.17325454611515614No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN502300.16743450537487095No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN488310.1627711393979758No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN479410.15980445196449708No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCNNNNN464150.1547177496909145No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN461960.15398774458087872No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN457320.15244106708746963No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN452100.15070105490738436No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN445170.14839103873727116No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN434690.14489768095043332No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN432530.14417767591039804No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN429910.14330433646368856No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTNNNNN429210.14307100149701046No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNN425160.14172099204694433No Hit
GTTGAAACCCATTAGTGATGGGGATCGGGGATTNN412390.13746429558340242No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN367500.12250085750600255No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN351020.1170074857190667No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGANNNN316780.10559407249184079No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATATATACGAAAAACAAAA314100.10470073290513034No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN313170.10439073073511514No Hit
CCTCAGATCAGACGTGGCGACCCGCTGANNNNNNN307530.10251071757502303No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN304090.1013640428816335No Hit
TTTAACCCCATTCGTGATGGGGATCGGGGATTNNN300880.10029403539158109No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTN300660.1002207015449108No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCAAAGNNNN300230.10007736720823712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)