FastQCFastQC Report
Tue 19 Sept 2023
SRR18017742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32797299
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN22729906.930418264016193No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN20412326.223780805852336No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN12783333.897677671566796No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN6159211.8779625724667144No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN5584781.7028170520993209No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN4776591.4563973697956043No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN4409431.3444491267405891No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN3522931.0741524782269418No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2836290.8647937746336978No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2823840.860997730331391No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2787390.8498840102656015No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2580520.7868086942159476No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2501830.7628158648064282No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2429780.740847592358139No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2326450.7093419491647773No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN2053070.6259875241555715No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1934670.5898869903890561No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1856190.5659581906424672No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1828920.5576434815562099No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1650410.5032152190337381No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1620380.49405897723468023No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1603490.4889091629161292No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1461970.44575926816412537No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1453240.44309746360515845No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1427930.43538036470625213No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1255520.38281201144033233No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTN1156550.35263574601066994No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1035560.3157455130680121No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN959280.29248750026640913No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN886740.2703698252712822No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN852340.2598811566769568No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN821520.25048404138401764No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN816640.2489961139787761No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN810570.2471453518169286No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN796240.24277608957981572No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN742790.22647901584822577No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN738530.2251801284002076No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA723890.22071634618448305No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN697460.2126577557499476No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN688560.20994411765432267No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN657930.20060493396117773No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN625690.19077485618556575No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN618330.18853076895143103No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN612480.1867470854840821No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN601850.1835059649271728No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN594900.1813868879873309No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN549550.16755952982591646No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN549060.16741012727907867No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN537130.16377263261831407No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN492990.1503142072766419No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN492880.1502806679293926No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN487940.14877444633474238No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN478930.1460272688918682No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN471650.1438075739102784No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN448880.13686492902967407No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN441750.13469096952160603No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN433140.13206575334145657No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCNNNNN422090.12869657345868632No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN413620.1261140437204905No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN403220.12294305088964795No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN386270.1177749423816882No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN379320.1156558654418463No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN373550.11389657422704229No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN365430.1114207605937306No Hit
TGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN363570.11085364072206069No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNN362970.1106706988279736No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN331280.10100831778860815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)