FastQCFastQC Report
Tue 19 Sept 2023
SRR18017743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27102506
Total Bases988.6 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN385756614.233244704382686No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN21651087.988589689824255No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN14698765.423395165007989No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3905861.4411434868790363No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3676041.3563469001722572No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3156521.164659828873389No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN3071071.1331313790687843No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN2064320.7616712639045261No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2040600.7529193056907727No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1872690.6909656250975463No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1535870.5666892943405307No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1516570.5595681816286656No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN1507660.5562806627549497No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1476010.544602775846632No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1466040.5409241492281194No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1371440.5060196278528636No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1354310.49969917910912004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN1331060.4911206365935312No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1308310.4827265788632239No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1258560.46437034272771677No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN1196320.4414056766558782No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1129160.41662568029686997No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1094040.4036674689786989No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1050310.3875324296579807No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1036140.3823041308431036No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1000610.3691946420010017No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN997230.3679475248524989No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN908820.33532692511902773No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN897660.3312092247115451No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN868360.3203984162940504No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN862710.31831373822036974No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA848450.31305223214414196No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN791520.2920467944919927No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN773700.2854717567445608No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN751200.27716994140689427No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN692610.25555201426761054No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN637930.23537675814922798No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN629070.23210768775404014No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN596250.21999810644816384No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN580850.2143159750614943No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN565530.20866336124046977No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN562990.20772617853123987No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN526790.19436948007686078No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN520870.19218518021912806No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN507280.1871708837551775No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN496910.1833446693084396No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN479780.17702422056469613No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN456320.1683681944392891No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN442010.1630882398845332No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN420070.15499304750638188No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN416990.15385662122904797No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN404730.14933305429394608No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGNNNN400250.14768007061782407No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTN386020.14242963362871316No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN366420.13519783004567917No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN358860.1324084200922232No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN358740.13236414374375563No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTNNNNN356640.13158930764557344No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN352940.13022412023449048No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN346100.12770036837183987No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN330690.12201454728946468No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN320570.11828057523536753No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN317010.11696704356416339No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN311480.11492664183895024No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN279850.10325613432204388No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN277410.10235584856987026No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN272490.10054051828270051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)