FastQCFastQC Report
Tue 19 Sept 2023
SRR18017744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33357708
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN27025018.101578801517178No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN18048595.410620537837912No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8769742.6289995703541744No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN7349782.2033228422048663No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN7244302.1717019646553655No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN6865482.058139006432936No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN6041611.8111586083792086No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN3928011.1775419342360092No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2978130.892786159049057No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN2416590.7244472551891156No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2372050.7110950188783953No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2163050.6484408341244547No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2071270.6209269533746143No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1912170.5732318299566626No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1889200.5663458652494949No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1740590.5217954423007719No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1696260.508506159955594No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1626160.4874915266960188No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1488040.44608580421652466No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1478440.44320790864887954No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1408950.4223761416701651No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1368460.41023801755204525No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1353110.40563638245169603No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1340870.4019670656029485No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1224720.36714752704232556No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1159320.34754186348774324No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1105950.3315425628163662No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1091770.3272916712383237No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1082880.324626620030369No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN981770.2943157845257234No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN937820.2811404188800981No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN911800.2733401227686267No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN866360.2597180837484398No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN821120.2461560008859122No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN810810.24306526095857667No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN786670.23582855272910236No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN739950.22182279429989615No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN677580.2031254665338518No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN651610.19534015946179517No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN648980.19455173598857572No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN648770.1944887820230335No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN647250.194033115224823No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCNNNNN634420.19018692771098064No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN627620.18814841835056534No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN626580.18783664633073713No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN610930.18314507699389898No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN596180.17872331036652758No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN588240.17634305090745442No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN559140.16761942996803017No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN544680.1632845997692647No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN519600.15576609759879184No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN491580.1473662399107277No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN484390.1452108160428768No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA475800.14263569907141102No Hit
TGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN452200.13556087246761678No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN438940.13158577921480696No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN411630.1233987658864332No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN398400.11943266605727228No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN391320.117310218076134No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN388140.11635691516935155No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN386590.1158922549474922No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN382090.11454324140015855No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN353060.10584060511591503No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN349180.10467745565732514No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN344260.10320253417890701No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN337670.10122697878403397No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN334700.10033662984279376No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN334020.10013277890675222No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN333590.10000387316778478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)