FastQCFastQC Report
Tue 19 Sept 2023
SRR18017745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535254
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN334499410.607157310354944No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN27951018.86341679696No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN10084123.197729119289795No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN9716793.0812467849474117No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN8831092.8003865134557024No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8107652.5709797675959734No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN6990382.2166873937340092No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN5232991.6594094977005733No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3406471.080210103904665No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2863970.9081804129435582No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2644750.838664562524215No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2344910.743583673053656No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2141320.6790241803665193No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2134320.676804442418634No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1861320.5902346624511094No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1658930.5260556962693245No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1428360.4529406993201957No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1384240.43895000814009616No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1251710.39692402667820587No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1232330.39077852361677506No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1226060.3887902726263121No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1219180.3866085873289621No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1206430.38256549320959965No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1184480.37560502921587374No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1174280.3723705539203838No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1002180.3177967109445194No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1001630.31762230296289984No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN991110.3142863539326495No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN985160.312399576676947No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN896100.28415816787142417No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN883800.2802577711915687No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN876360.27789850685838774No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN809560.25671586472714No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN762440.24177385728366102No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN740530.2348260775067802No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN729270.2312554704648962No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN715210.22679696824385812No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN689290.2185775957282602No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN665790.21112561833178828No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN632010.20041379720613633No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN609580.19330112261026977No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN574650.1822246302503224No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN566410.17961168158024032No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN541010.17155720388362813No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN529280.16783755729381472No Hit
CCCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN501230.1589427502312174No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN489080.1550899193645309No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN458220.14530404606856823No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN448230.14213616291151482No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN441750.14008131978261534No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN402030.1274858924554722No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN395240.12533274664602354No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN392860.12457803574374256No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN380250.12057933638333783No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN375080.11893990135611401No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN361190.11453530705666745No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN360620.11435455696662536No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN358050.11353959603433034No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN350220.11105666058691013No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNN344820.10934429131282722No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN335200.10629373716159063No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN331790.1052124076755494No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN328460.10415644662319827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)