FastQCFastQC Report
Tue 19 Sept 2023
SRR18017746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34276147
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN509626114.868243504732314No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN28618138.349284416361034No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7138992.0827866095917957No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3654311.0661379180104462No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3579771.0443910162948011No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3340420.9745611138848248No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2749620.8021963495488569No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN2583060.7536027897184593No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2568920.7494774718990439No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2548380.7434849663820149No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN2511230.7326465252935227No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2357060.6876677241464743No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN2079820.6067834870704691No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN1879640.5483813568660445No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1739080.5073732470572028No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1732720.5055177292826991No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN1649300.48118010463661504No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN1598510.4663622197675836No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1550750.4524283315741411No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1549550.4520782338808385No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1305980.38101715458274815No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN1282800.3742544341404534No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1256730.36664856175345495No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1221810.35646071887835No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1214340.35428136073754146No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN1094630.31935619834983203No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1076120.31395594143063976No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN1047020.30546607236805234No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1032530.3012386427214237No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1020810.2978193552501686No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN992830.2896562440346635No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN977400.2851545711949479No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN920650.2685978677825136No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN911990.26607132942918No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN891100.25997671208493767No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN858430.250445302384775No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN838620.244665772964505No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN741340.21628451996077622No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN731020.21327367979837408No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN657580.19184770096825646No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN650900.1898988238088721No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAANNNNNNN642790.18753274689830218No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN634500.1851141553337369No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN623800.18199245090178895No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN587450.17138740827549842No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN567330.16551743695112522No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN564460.16468011996797655No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN526930.1537308146099385No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN526670.15365496010972296No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGNNNN519460.15155145646912999No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN514880.15021525027302515No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN491530.14340293265751253No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN486430.14191501746097657No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN478400.13957228039662684No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN457450.1334601581677194No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN454710.1326607684346785No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN448540.13086068279494775No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN444590.1297082778878268No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN441410.12878051900057497No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN437660.1276864637090044No Hit
GGCGGGGCGCGGGACATGTGGCGTACGNNNNNNNN433310.12641735957078257No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN416560.12153057926843412No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN416070.12138762271033557No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA406180.11850223422136683No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAANNNNNNN405990.11844680208659392No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN374960.10939385923394482No Hit
TTAACCAGTGAAATTGACCTGCCCGTGANNNNNNN367370.10717949132380603No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN365820.1067272818032902No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGNNNNNNN364420.1063188344944372No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGANNNNN358380.10455667610481424No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATANNNNNN355750.10378937866032609No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGNNNNNN355500.10371644164088804No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAANNNNNN352260.1027711778689711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)