FastQCFastQC Report
Tue 19 Sept 2023
SRR18017748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21351752
Total Bases770.9 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN229145010.731906215471218No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7429663.479648883145514No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN6689993.1332276620672626No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN6001802.810916874643355No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN5621232.6326785736364866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4986912.3355975659514967No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN3561171.6678584502105496No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3373281.5798609875199001No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN3128311.4651303555792516No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3052121.42944710110908No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN2444371.1448100371341892No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2274761.0653739327807854No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2274451.0652287456317402No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN1528770.7159927672445802No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1471480.6891612454097443No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN1452180.680122174517576No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN1365420.6394885066106051No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1359700.6368095695379002No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1269190.5944196054731246No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN1114990.5222007074641931No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1111980.5207909870815285No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1057380.4952193150238912No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN971430.4549650070870063No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN928430.43482614447751167No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN892870.4181717734451018No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN834240.39071266844987707No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN829410.3884505589986246No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN804340.37670913375164716No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN716800.335710156243853No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN710250.3326424922882207No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN669960.31377284636876634No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN651400.30508035125173805No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN633500.29669696425848335No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN593300.27786946944681634No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN567860.2659547563122689No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNN553460.25921057906629863No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN537890.2519184374190933No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN537150.2515718616439532No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN520500.24377390670330001No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN505650.2368189739183932No Hit
TGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNN494340.23152198470645408No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN486400.2278033203083288No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN465050.21780414084989372No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN450650.21105996360392346No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN445940.2088540556297207No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN431970.2023112670098454No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN426920.19994612151733498No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN416730.19517367942452685No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN406500.19038250350603547No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN403090.18878544486653834No Hit
CAACTTCTTAGAGGGACAAGTGGCGTNNNNNNNNN390190.18274378608369No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN386970.1812357131161883No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN371910.1741824277464444No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN368120.1724073977629564No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN366010.17141918845816492No Hit
GGAGATACCATGATCACGAAGGTGGTNNNNNNNNN363950.17045439643547752No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGANNNNNNNN361770.16943340293574036No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN352980.165316644741846No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGNNNNNNN332180.15557505538655564No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN331550.15527999763204442No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN315670.1478426688357939No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN306020.14332313338970967No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN302630.14173544166305418No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN290630.13611529395807895No Hit
GGGAGGAGGGGAGGAGGCGTGGGGGGNNNNNNNNN287950.13486012763730115No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN287170.13449481803647775No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATANNNNNN272230.12749773414378363No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN265130.1241724800850066No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN260680.12208834197774496No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAANNNNNNN252560.11828537536404506No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN249780.11698337447905915No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN249540.11687097152495964No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAANNNNNNN246370.11538631583956202No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAANNNNNN242580.11361128585607402No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN231740.10853441909591308No Hit
CCCCTTGTAAATTTAACTGTTAGTCCANNNNNNNN229900.10767266311448352No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN229240.10736355499070989No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN228940.10722305129808553No Hit
ACCTCTAGCATCACCAGTATTAGAGGNNNNNNNNN227970.10676875602526668No Hit
CGCGCGCTACACTGACTGGCTCAGCGNNNNNNNNN218080.10213681762508293No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN214940.10066621230894776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)