FastQCFastQC Report
Tue 19 Sept 2023
SRR18017749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29652731
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN497658616.782892611139257No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN21238327.1623487226185No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN7353092.4797344973048183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6980672.3541406692017675No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN6765472.2815672526082No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN5788891.9522282787376313No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5105131.7216390625200761No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN4554681.5360069195650141No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN4550381.5345568001814065No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3903931.3165498988946414No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3254481.0975312864100106No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3184031.073772935113464No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2961000.9985589522934667No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2648360.8931251559932203No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2419040.8157899520283646No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1988120.6704677555669324No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN1808000.6097246152470746No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1711830.5772925266141591No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1666690.5620696454569396No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1370210.46208560014252986No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN1314530.4433082403101421No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN1121450.37819450761550427No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN1059470.3572925542675985No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1032420.34817029163350927No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN999520.33707519216358184No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN958600.3232754514246934No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN956890.32269877604190994No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN901340.30396525702809635No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN898740.30308844065661267No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNN853970.2879903372137966No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN813320.2742816504827161No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN813170.2742310649228228No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN789170.266137375339897No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN788810.2660159699961531No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN758090.2556560473300082No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN755900.2549174981555662No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN749720.2528333730879628No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN730980.24651355047196158No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN700390.23619746862439078No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN680370.22944598256396687No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN603520.20352931404530664No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN563210.1899352879166509No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN506050.17065881722664938No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN497780.16786986669119955No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN489570.16510115037970702No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN467760.15774600997122323No Hit
TGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNN461480.15562816119702433No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN454070.153129234538296No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN453680.15299771208257346No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN425880.14362252164901776No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN418840.14124837270469287No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN414260.13970382694261785No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN399310.134662132806587No Hit
GGAGATACCATGATCACGAAGGTGGTNNNNNNNNN382070.1288481657895187No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN371180.1251756541412661No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGNNNNNN368430.12424825220988919No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN367910.12407288893559248No Hit
CAACTTCTTAGAGGGACAAGTGGCGTNNNNNNNNN365600.12329387131323588No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN362220.12215401003030715No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN361490.12190782697215984No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCNNNNNNN360430.12155035568224729No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN347240.11710219878229766No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN346690.11691671839602227No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN342270.11542613056450013No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN327280.11037094694583106No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN323470.10908607372454159No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN314650.10611164280281636No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN309240.10428719027599852No Hit
GCATGCTCATAAGGAAAGGTTAAAAANNNNNNNNN305790.10312372239845294No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN300520.10134648306086884No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN300500.10133973831954973No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN299130.10087772353919104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)