FastQCFastQC Report
Tue 19 Sept 2023
SRR18017750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30839652
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN19268166.247852602227807No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN18976316.153217941629173No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN16521865.357343202186588No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN13335024.323985238225127No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN10963013.5548423179353645No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN9067342.9401563934638433No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN6216262.0156712533591494No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN5147731.669191986991293No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4980871.6150863180946398No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN4429051.4361543379283268No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN4388331.4229505572890382No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3654381.1849614904863388No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN2790240.9047572910355798No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNN2784850.903009541093395No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN2259140.7325439340236394No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN2149510.6969955432700732No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN1949310.6320791168460657No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN1683290.5458200371391999No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1592550.5163968776301366No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN1586500.5144351174909497No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN1503390.48748604556238184No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN1214620.39385009921642433No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1155880.3748031916832265No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNNN1026570.3328734059645031No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN1008690.32707567517298836No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNN966860.3135119682932868No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGNNN934280.30294764675035896No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN921390.29876796275133066No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN888160.287992873590143No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN887860.28789559622786925No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN821620.266416754637828No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN819830.265836333042928No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNN803720.2606125386888283No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN768940.2493348498225596No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN761750.24700343570673236No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNN665850.21590710556656087No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNN654260.2121489568040521No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGANN639890.2074893711511401No Hit
GGAGATACCATGATCACGAAGGTGGTNNNNNNNNN604710.1960819791351731No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGANNNN597570.19376677791305816No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN594960.19292046486127665No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN588520.19083224415113373No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGNNNNN583570.18922716767361705No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN568760.18442490855603688No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN566140.1835753529255129No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCNNNNNNN560730.18182111782584318No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGNNN554540.17981396158426172No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNN551380.178789306701645No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN547290.17746309199597973No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNN538600.17464529106878376No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN524130.16995327962844717No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNNN513360.16646102232281998No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN506860.16435334614022232No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNN499080.16183061987859007No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN494240.16026121176724042No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN485110.15730073737537636No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN481680.15618853286671328No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN479180.1553778881810988No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN470690.15262493882875203No Hit
CCCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN469550.15225528485211182No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN468460.15190184376918392No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN467330.15153543237128617No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN461270.14957042965335665No Hit
TGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN461040.14949585034228013No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN457920.14848416577463328No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN430280.13952167813047955No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN415540.13474211706409656No Hit
GGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN411810.13353263519315978No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN405940.13162924147133698No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN405450.13147035511295654No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN396290.12850015298486506No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN394870.12803970680343604No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN387040.12550076764809148No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNN385590.1250305937304351No Hit
GAGTTCAATGGCTGAGGTGAGGTACCCNNNNNNNN378930.1228710362879581No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNN378470.12272187766580506No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGNNNNNNN369900.11994298768351862No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN366040.11869135228892984No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNN365880.11863947102905051No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN363360.11782234118595113No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN353970.11477755974678312No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA353860.1147418913806161No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNN348530.11301359691088603No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN346290.11228725927257546No Hit
CGACTTCATAATTTGTGGTAGTGGGGGNNNNNNNN345640.11207649165431569No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNN344970.11185923887857101No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN342680.11111668834654813No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTNNNNNN325150.10543244781101939No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNN319830.10370739592003178No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN318020.10312048916764689No Hit
CCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN308630.10007570772847892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)