FastQCFastQC Report
Tue 19 Sept 2023
SRR18017751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26731371
Total Bases955.4 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN22770308.518193847969863No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN20624737.715552636638054No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN14799385.53633406980884No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN11280874.220086579173212No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN6452322.4137632147636574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4958971.8551124818850482No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN4958381.8548917674293623No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN4184031.565213396649203No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN3167231.1848363482740936No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2258960.8450595369762367No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN2246460.8403833832540799No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN2113980.7908236356451751No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNN2022700.7566764907044985No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNN1991010.7448215057880869No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN1904070.7122979214197431No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN1902230.7116095915918417No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN1654390.6188945565118975No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN1591430.595341705444139No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN1523380.5698847245807183No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN1426640.5336950356942036No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1327300.49653270683348044No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGNNNNNNN1014060.3793520354792128No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN1005000.37596275926139366No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN911380.3409402383439293No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNN883260.33042076293056577No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN846430.3166429436036034No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNN824770.3085401044338504No Hit
GGAGATACCATGATCACGAAGGTGGTNNNNNNNNN706640.2643485812979813No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN693290.259354449122718No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN682860.25545266645695053No Hit
TGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN667020.24952704446023363No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNN641800.24009243671041042No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNN641210.2398717222547246No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN631310.23616820850677656No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGNNN627640.23479528977395137No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTNNNNNN622320.23280511874980148No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN607040.2270889884398372No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGNNN604450.2261200893886064No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNN591310.2212045165958753No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN560020.20949916859857284No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN533760.19967550485906616No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGNNNNN526790.19706808154359162No Hit
TCGACTGCATAATTTGTGGTAGTGGGNNNNNNNNN526180.19683988524195037No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGNNNN518560.19398930193292369No Hit
TGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNN512620.19176719368415485No Hit
GGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN511080.19119109154558514No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNNN507980.1900314054224903No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN495520.18537021539224455No Hit
ACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNN490970.18366809543737955No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTNNNN484730.18133375949927896No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCNNNNNNN480760.179848613077122No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNN478550.17902186909904472No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN474310.1774357177564892No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNNNN472920.17691572946258535No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN471040.176212435942773No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN469180.1755166242689161No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN453730.1697368982683305No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGNNN450400.16849117091674795No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNN448010.1675970903250716No Hit
CCCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN412550.15433177744605767No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN412450.1542943682162804No Hit
CGACTGCATAATTTGTGGTAGTGGGNNNNNNNNNN405340.15163457197911773No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNN400800.14993619294723043No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNN399020.14927030865719534No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN388530.14534608045356148No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN379180.1418483174693883No Hit
GAGTTCAATGGCTGAGGTGAGGTACCCNNNNNNNN374500.14009756551581284No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGNNNNN370500.13860119632472273No Hit
GCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN354480.13260823771440677No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGNNNNN352520.13187501681077263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN351560.131515888204911No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNN341370.12770388769060892No Hit
CGACTTCATAATTTGTGGTAGTGGGGGNNNNNNNN328990.12307262504418497No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNNN319710.11960104852085589No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNN312610.11694499320667091No Hit
CCACATGGTCTAGCGGTTAGGATTCCTGGNNNNNN305040.11411311451253285No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTNNNNNNNNN303740.11362679452542856No Hit
CCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN299660.11210049795051666No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN293380.10975119832050514No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNN291000.10886085865180653No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGNNNNNN290110.10852791650678897No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGANNNN287130.10741312145942682No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN283570.1060813528793566No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN282950.10584941565473766No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN282580.10571100150456182No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGNNNN279570.10458498368826649No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGNNNNN278310.1041136273930731No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN277980.10399017693480818No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGNNNN272240.10184288714559385No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN268110.1002978859557933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)