FastQCFastQC Report
Tue 19 Sept 2023
SRR18017752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25445851
Total Bases905.5 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN312043912.26305616581658No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN15243975.990748747212266No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4286421.68452609425403No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3971111.5606119834624514No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3467311.362622928193677No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3435581.3501533118306792No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3122461.2270998521527143No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2426810.9537154013831175No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2376250.9338457574085458No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN2211310.8690257598380183No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN2181670.8573774954510265No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2060290.8096762022225156No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1894960.7447029380153173No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1834080.7207776230396068No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1657170.6512535186974097No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1524560.5991389323155276No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1395520.5484273251462488No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1049950.4126212953145092No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1012250.39780552043631784No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1006150.3954082730422339No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN1003540.39438256555066675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN840100.3301520550442585No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN815390.32044123814133785No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN813740.31979280237080693No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN802120.3152262425807649No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN727140.2857597492023356No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN718180.2822385464726646No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN697260.2740171668850847No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN693510.2725434492247872No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN643260.25279563257680004No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN640620.2517581353439506No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN603820.23729605270423065No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN591580.23248583826101943No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN553460.2175050070048748No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN525250.20641872028567643No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN523460.20571526572249443No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN490770.1928683776384606No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN483070.18984234404264963No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN477110.18750011544121672No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN472340.18562554657731825No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN460220.18086249109923658No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN440930.173281687454666No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN429780.16889983361138128No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN419050.1646830361460499No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN403800.15868991766083987No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN394930.15520408415501608No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN394740.15512941579356102No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN393370.1545910176083323No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN386240.15178898909688657No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN381550.14994585954307443No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN368040.14463654605224247No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN366060.14385842312760536No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN364120.14309601985801143No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN356680.1401721640199811No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN355890.1398617008328784No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN339610.13346380123030666No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN325250.12782044506980728No Hit
GGCGGGGCGCGGGACATGTGGCGTACGNNNNNNNN316300.1243031722538971No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN312870.12295521183394495No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGNNNNNNN312510.1228137349385564No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCNNNNN302790.11899385876306513No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN299900.11785811368619584No Hit
CGGCGGGGCGCGGGACATGTGGCGTACNNNNNNNN297250.11681668653958557No Hit
GGCGGGGCGCGGGACATGTGGCGTACNNNNNNNNN286900.11274922579716433No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN271770.10680326627708385No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN266460.1047164820701025No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN264280.10385976087024953No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN263660.10361610621708033No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN260620.10242141243379914No Hit
CCTCAGATCAGACGTGGCGACCCGCTGANNNNNNN259140.10183978519720169No Hit
ATGATTAAGAGGGACGGCCGGGGGCNNNNNNNNNN254800.10013420262501734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)