FastQCFastQC Report
Tue 19 Sept 2023
SRR18017753_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017753_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27312241
Total Bases979.2 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN25592439.370314944130728No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN14968835.4806304616307395No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN6066212.2210590482121186No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN5834612.1362619054218217No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5131691.878897451146539No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4808891.7607086873610993No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3782601.3849467716691575No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2759491.010349169077704No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2759311.010283264562582No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2393440.8763250148532301No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1961690.7182457126092289No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1894980.6938207670326283No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1881140.6887534420921374No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1333070.48808517763152426No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1299480.47578666283737026No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1229460.4501498064549152No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1228040.44962989305784173No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1162180.4255161632470949No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1160480.4248937317153872No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1088870.39867471878268795No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN949010.34746691053289985No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN947950.3470788061660704No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN942570.345108993436313No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN941890.34486002082363No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN928280.3398769072080171No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN854070.31270594016800013No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN812850.29761380620506384No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN799550.29274419480993885No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN749450.2744007714343177No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN678100.24827695391235016No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN675740.2474128724918618No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN646540.23672169559429418No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN580350.21248714083915704No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN578330.21174754572501026No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN577230.21134479591037586No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN567570.20780792026549563No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN525280.19232402057377862No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCNNNNN520310.19050432368402137No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN518980.1900173625445089No Hit
GGGGGGGGATGCGTGCATTTATCAGANNNNNNNNN497470.1821417729874308No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN494630.18110194619328382No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN465650.17049131925864305No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN448670.16427432666546843No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN446710.16355669972302894No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN437050.1600198240781487No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN420280.15387972008594974No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN410320.15023300358253283No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN402850.14749796620497016No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN402540.14738446398448227No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN363850.13321865459520513No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN347230.12713347103227451No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN330310.12093844661080723No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN330280.12092746252495355No Hit
CCTCAGATCAGACGTGGCGACCCGCTGANNNNNNN327780.1200121220371481No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN320850.11747479820495141No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN306270.11213653248007002No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN305710.1119314962108016No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN298350.10923673381470235No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA293140.1073291642381158No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNN287110.10512136298152905No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTNNNNNN287050.10509939480982172No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN279030.10216298252494183No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN274090.10035426972103828No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN274070.10034694699713581No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN273940.10029934929176995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)