FastQCFastQC Report
Tue 19 Sept 2023
SRR18017754_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017754_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30353059
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16500035.4360352938397405No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN12419804.091778690246673No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN7503622.472113272009915No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7355972.423469080991145No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN5980351.970262700705059No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN5931751.954251134951505No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN4997671.6465127946412255No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTNNNNNNNN4485171.4776665508408888No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN3646521.2013682047664456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN2294300.7558710968802189No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2065540.680504722769458No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1700010.5600786398497759No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1674930.551815881226337No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1312520.4324177012933029No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1305780.4301971672772751No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN1266350.41720671382742675No Hit
AGACGACCTGCTTCTGGGTCGGGGTTTCGTNNNNN1238880.40815655515972876No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1079660.3557005572321393No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN1027270.33844035291467656No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN964350.3177109760172772No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN846200.2787857395196972No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN842680.27762605409886365No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN840080.27676946827665705No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN814740.2684210510709975No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN811880.26747880666657026No Hit
AGCGTTTACTTTGAAAAAATTAGAGTNNNNNNNNN770230.25375696070699166No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN741860.24441029156237595No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN709900.23388087507094424No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN705490.23242797373404772No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCNNNNN680550.22421133896257375No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN676070.2227353756996947No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN638970.21051255492897766No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN617100.20330735033987843No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN582270.19183239488316484No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN559970.18448552417731603No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN555130.18289095672366992No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN550330.18130956751344238No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN520970.17163673684421726No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN462340.15232072655345874No Hit
AGGAAAAAACCTTGTAGAGAGAGTNNNNNNNNNNN459400.1513521256621944No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTNNNNNN456260.1503176335538372No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCNNNNNN455900.1501990293630701No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN455400.1500343013203381No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN455020.14990910800786175No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN451750.14883178660839422No Hit
ACCCAATTGGACCAATCTATCACCCTATNNNNNNN445460.14675950783082523No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN443850.14622908353322808No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN440490.1451221110860688No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN415620.13692853824057732No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN410660.13529443605667552No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN401130.13215471956220293No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN377600.12440261787123334No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN377010.12420823878080954No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTNNNNN374280.12330882366749261No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN359650.11848888113715325No Hit
CTCCCGGGGCTACGCCTGTCTGAGNNNNNNNNNNN347760.11457164828098546No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCNNNNNNNNN344540.11351079968579113No Hit
GGGGGGGGATGCGTGCATTTATCAGANNNNNNNNN342360.11279258541947947No Hit
GGAGGTGGAGCACGAGCGCACGTGTTNNNNNNNNN341990.11267068666785775No Hit
ACTTCGAACGCACTTGCGGCCCCGGGNNNNNNNNN336060.11071701208105582No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTNNNNNNNN328470.10821644039238351No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGNNN306380.1009387554644822No Hit
GACTCTAGATAACCTCGGGCCGATCGCACNNNNNN304480.10031278890210045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)