FastQCFastQC Report
Tue 19 Sept 2023
SRR18017756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32164341
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN448356013.939536333108768No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN22149896.886474061445873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15377594.780943592160026No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN10390253.23036309060397No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5064641.574613327224705No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3995031.242068040504856No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN3963071.232131570797611No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3842351.1945993235179293No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3220741.0013387185517029No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2703380.8404897833908677No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2648240.8233465750161024No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN2518010.7828576372822313No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2305230.7167036315154102No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1591020.4946533802760019No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN1588870.49398493816490757No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN1546340.480762220497538No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1317220.40952805468639947No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN1279180.3977012928696409No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN1279080.3976702025388924No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1233530.38350855688291574No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1142220.35511997587639055No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1122470.3489796355535467No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1114900.34662609751587947No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN1109610.34498141901927976No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN960090.2984951564840082No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN934530.290548467944672No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN919900.2859999525561553No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN908250.2823779290239461No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN811160.25219232690015314No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN772850.24028162119037352No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN725240.22547951472097627No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN698930.21729964870102575No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN691680.21504559972175397No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN639880.19894080839399134No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTN614610.19108428181382606No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN611680.19017333512289278No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN588470.18295726935614817No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN574220.178526897224476No Hit
GTTTAAACCCAATACTTAATGGGAAACGGGAATAAATACGAAAAACAAAA568810.17684491033097802No Hit
GGGGGGGGATGCGTGCATTTATCAGANNNNNNNNN543070.16884225919629445No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN526890.16381184368117477No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN523610.16279208083262145No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN513610.1596830477577638No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN497490.15467128644109326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN470990.1464323487927205No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN461960.14362489192612402No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN461430.14346011317315657No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCNNNNN461380.14344456800778227No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN448610.13947433277118906No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN437430.1359984337934982No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN426230.13251631674965764No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN415940.12931712171562912No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN413270.12848700988464212No Hit
GGGAGGAGGGGAGGAGGCGTGGGGGGNNNNNNNNN410270.12755429996218484No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN407940.126829895255743No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN402800.12523185225526617No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGANNNN392890.12215080047808223No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN392180.12193005912976733No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNN386690.12022319997167048No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCNNNN372590.1158394633361212No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN366610.11398026155735634No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN358670.11151168929591936No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNN351820.10938200163964186No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN345420.10739222047173297No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN343520.10680150418751No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN342030.10633825825935622No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGNNNN337600.10496095660719428No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN323870.10069225419541473No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGNNNN322710.10033160635873124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)