FastQCFastQC Report
Tue 19 Sept 2023
SRR18017757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21009133
Total Bases749.6 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18985099.036588992035036No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN9984694.752547380227447No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4389192.089181881041926No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN3727801.774371174669607No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2759891.3136620154672733No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2707841.2888870759207436No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN2653171.262865059686185No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN2140631.0189044926318473No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1728650.8228088231913235No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1352610.6438199996163573No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN1333840.6348857899086079No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN1131750.538694290716328No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1087340.5175558648707683No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN966850.4602046167254974No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN951800.45304106552136153No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN829290.39472833076928976No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN664130.3161149010765937No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN650000.3093892546636741No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN563980.2684451566849522No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN528430.2515239443721928No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN491730.23405535107041303No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN467260.22240803559099748No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN433440.20631027467911217No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN412060.19613374811802087No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN403640.1921259673114545No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN399070.1899507228594345No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN390640.18593818221818104No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN369180.17572357697959265No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN361120.1718871502217631No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN353090.16806500296799493No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN351020.1670797171877583No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN344530.1639905844758087No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN320620.1526098197388726No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN287910.1370404004772591No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN287660.1369214046100808No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN286530.13638354329043467No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN285210.13575524511173306No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN282100.13427493652403455No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN280870.13368947685751714No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN280010.13328013107442369No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN276790.1317474643051667No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN256950.12230395228589395No Hit
GGGAGGAGGGGAGGAGGCGTGGGGGGNNNNNNNNN242030.11520227893269085No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN239630.11405991860777881No Hit
GACTGCATAATTTGTGGTAGTGGGGGANNNNNNNN236060.11236065762447217No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN234260.11150388738078816No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN232330.1105852392861714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN228130.10858610871757535No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN218940.1042118206400997No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN211410.10062766512068821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)