FastQCFastQC Report
Tue 19 Sept 2023
SRR18017758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43370799
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22058775.086087992061202No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN19928474.59490497281362No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN11341832.615084402756795No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN11288552.602799639453264No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN11029892.5431604338209217No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7645821.762895813840091No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN6754101.5572920388208666No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN5071391.1693097929784508No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN3256620.7508784885424868No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN3160640.7287483912851134No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN3073940.7087579825310574No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2773490.6394832615373307No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2678340.6175445372818702No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2203550.5080722630911181No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2197840.5067557090659086No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN2084100.48053069070735815No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN2015170.46463750875329735No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNN1976240.4556614232539271No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN1681220.3876386967184995No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1434950.33085625192194407No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN1305190.30093750405658887No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNN1301690.30013050947020825No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN1297790.2992312869310985No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN1287670.2968979197270495No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN1192260.27489924730231513No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNN1126910.25983150552518064No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGNNN1116890.257521195309314No Hit
ACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNN1033560.23830780705700166No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN1024250.23616120145722932No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN951610.21941260524160508No Hit
GTTGAAACCCATTCGTGATGGGGATCGGGGATTNN931080.21467900556777844No Hit
GGGAGGAGGGGAGGAGGCGTGGGGGGNNNNNNNNN918770.21184069032253705No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN837350.19306769054450668No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN812020.18722735543792957No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN780710.1800082124380508No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN763200.17597093380732967No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN757910.1747512191324859No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTN720660.16616249103457836No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN712850.16436174025754058No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN711230.16398821704898728No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNN701650.16177935758112272No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN698990.1611660416954735No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN694170.16005469486508653No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNN688240.15868741546587603No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN653270.15062438669852496No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN653100.15058518981861505No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN650050.14988195167905485No Hit
TTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNN649340.1497182470629605No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNN617230.1423146481576233No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN613700.141500736474788No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN607550.14008273170157645No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN560230.12917216489371108No Hit
GACCTGGTATTGCAGTACCTCCAGGNNNNNNNNNN554680.12789250204959332No Hit
ACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNN549970.1268065179062069No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN542720.12513488626299No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN525140.12108146774054128No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNN521020.12013151982743044No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN504880.11641012193480686No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN504050.11621874893289377No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNN485320.11190017504634857No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCNNNNN483390.11145517517443014No Hit
CTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNN465610.10735564267561684No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCNNNNN460920.10627426992986687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)