FastQCFastQC Report
Tue 19 Sept 2023
SRR18017759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR18017759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39737979
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN398395710.025565215583812No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18058914.544496336867056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN8133322.04673720321811No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5891591.4826093697417273No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNNN4794681.2065736911280767No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN4226241.0635266579611409No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNN4142221.0423831569290427No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN4109551.0341618027429125No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2818960.7093868563371076No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN2558180.6437619789370768No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN2418350.6085739790642096No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN2291380.5766221779924943No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNNN2240200.5637428113795118No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2075350.52225856780487No Hit
GGCATGCTCATAAGGAAAGGTTAAAANNNNNNNNN2064310.5194803691450942No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNN2042330.5139491366684752No Hit
TGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNN2019640.5082392338070338No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1998240.5028539574194248No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1971350.49608713115480785No Hit
GACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNN1845200.4643416817951411No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1752830.441096916378158No Hit
GGGAGGAGGGGAGGAGGCGTGGGGGGNNNNNNNNN1702480.42842641796151737No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTNNNNNNN1526310.38409351416688803No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNN1408880.3545424391109573No Hit
GGCATGCTCATAAGGAAAGGTTAAAAANNNNNNNN1388150.3493257671709978No Hit
GCACGAGCGCACGTGTTAGGACCCGAAANNNNNNN1307340.32899005759704086No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN1278270.3216746377564898No Hit
ATGTACACGGAGTCGACCCGCAACGCACNNNNNNN1269590.31949032938992694No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACNNNNNN1224940.3082542270204532No Hit
ACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNN1203580.3028790165700173No Hit
TGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNN1109960.2793196906163748No Hit
AGGCATGCTCATAAGGAAAGGTTAAAANNNNNNNN1105150.27810926167130945No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN1099420.2766673161712628No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNN1065860.26822199488303117No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNNNN1059730.26667939001125346No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGNNNNNNN937060.23580967718564652No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN921390.23186634629808428No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN869990.2189316170306497No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCANNNNNNN833160.20966340537851713No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNN823400.20720731670828052No Hit
CACTCGCCGATTCCCGGGGCCGAGGGAGCGNNNNN811630.20424541469509558No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN757390.19059600388837086No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN737910.1856938924850708No Hit
CGCCACGCAGTTTTATCCGGTAAAGCNNNNNNNNN735780.18515788133060315No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN729720.18363289184887838No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN726350.18278483664204462No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNN695890.17511962548472834No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAANNNNNNN623800.1569782902145074No Hit
CCACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNN622840.15673670772235299No Hit
ATGTACACGGAGTCAAGACCCGCAACGCACNNNNN622330.15660836702339592No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNN613280.15433094873798187No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN585420.14732002349691714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN583380.146806660701089No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN577950.14544020972984056No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN572090.1439655499339813No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN561590.14132324142604233No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN548900.1381298228578761No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN545080.13716852585784495No Hit
CGGCGGGGCGCGGGACATGTGGCGTACNNNNNNNN517430.130210446786939No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTNNNNN503780.12677544572661836No Hit
CAACTTCTTAGAGGGACAAGTGGCGTNNNNNNNNN476430.11989286118451066No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN466730.11745187142003372No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTNNNN452490.11386839778640982No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNN447950.11272591391726287No Hit
GGCGGGGCGCGGGACATGTGGCGTACNNNNNNNNN440910.11095430897479713No Hit
CACGAGCGCACGTGTTAGGACCCGAAANNNNNNNN424160.10673919778356115No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGANNNNNNNN423400.10654794497727224No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNNN405430.1020258227022567No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGANNN402580.10130862467867326No Hit
CGCGCGCTACACTGACTGGCTCAGCGNNNNNNNNN397460.10002018472051637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)