FastQCFastQC Report
Fri 30 Jun 2023
SRR1802134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16768078
Total Bases855.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT230970713.774429007307814No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4067352.425650691748929No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA2740591.634409143373498No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA2652501.581874798053778No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2317271.3819532566582766No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA2007011.1969231059158956No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1503740.8967873360321916No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1494310.8911635549405245No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1187370.7081133568200244No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1130440.6741619403249436No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1066520.6360418886410237No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG975110.5815275906994231No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA962570.5740490949529218No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA898310.5357262770366407No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA883470.5268761273653426No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG843470.5030212764993102No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA700610.41782367663127523No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT689230.411036971559889No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC685860.40902720037442575No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA592030.3530696839554301No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG588590.35101816678095127No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC531310.3168580203407928No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG525650.31348255894324917No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT525300.3132738289981714No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG503720.30040413695594687No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC500590.2985374948756799No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC492210.29353990361924603No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC480770.2867174162715608No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG478440.28532787120861436No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT466160.27800443199274244No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT459000.27373441368772256No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG458280.273305026372134No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG455910.27189162645832154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA452680.26996534725088944No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG447540.2668999989146043No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA420540.2507979745800324No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG419030.24989745395983964No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT414670.24729727521544212No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG403360.2405523161330714No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAG386270.23036033110055906No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG376690.22464709431814425No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGATC364090.21713281629534403No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT364070.21712088886991104No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA349260.20828863033676254No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAGAT348990.20812761009341682No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC335180.19989172283191906No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAGA335020.19979630342845495No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG333110.1986572342996019No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATC326670.19481660331017067No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC313010.18667017173942058No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA307240.1832291095019954No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG300330.1791081840148883No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT297790.17759340098489523No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA295640.17631120275084597No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA292400.17437895983069734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA291430.17380047969719606No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC282290.16834964627430765No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC279920.16693624636049523No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG272780.16267815548090842No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA272080.16226069559075287No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG266710.159058181861988No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC264000.15744201571581432No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC259620.15482990954598375No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA253090.15093560514210394No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC252790.1507566937606087No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGA247720.14773309141333907No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT245440.14637336491397523No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT241300.14390438784934087No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA238760.14238960481934781No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG234790.14002201087089408No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA233340.13915727252700041No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG232330.1385549375426331No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGA227770.13583548454390537No Hit
TAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG227670.13577584741674031No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC226570.13511983901792443No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATAG222810.13287748303651736No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA222140.13247791428451133No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA221560.13213201894695387No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATAG220310.13138655485739034No Hit
TGCACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGA216980.12940063852279313No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT212390.1266632943859159No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC210600.12559578980966096No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG208770.12450443038253996No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGA207600.12380667599470851No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA206020.12286440938550024No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTTGTAGGCACCATCAAT205150.12234556637916404No Hit
TGCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGAT203840.12156432001330147No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCACCATCAATAGA203820.12155239258786846No Hit
AGTGCGGTAACGCGACCGATCCCGGAGACTGTAGGCACCATCAATAGATC203110.12112896898499638No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG188250.11226689188826532No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA187360.1117361214564961No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG186070.11096680251606655No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAAT184120.10980387853634745No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAAT184070.1097740599727649No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACATCAATA182700.1089570313306033No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACCTGTAGGCACCATCAATAGATCG182380.10876619252367505No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAAT174000.10376860126724125No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG173570.10351216162043139No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGTAGGCACCATCA169620.10115649509741069No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA169010.1007927086217037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)