FastQCFastQC Report
Fri 30 Jun 2023
SRR1802135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1802135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22992074
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT238351310.366672445469687No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA4621882.010205777869365No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA3973041.7280041809190418No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3049971.3265310471773881No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2671751.162030880728724No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA2346851.0207213146582599No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA1962150.8534027856730106No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT1881650.8183907202107996No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1801730.7836309155929125No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1719760.7479794993700873No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1593990.6932780400758976No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1548680.6735712489443101No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1408090.6124240901451518No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1368530.5952181608322938No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1340710.5831183389545458No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1277200.5554957765010673No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT1129320.4911779598482503No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAG1038530.4516904390617393No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1021440.4442574428039854No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG1003840.4366026309762225No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC909630.39562764107318027No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG862630.37518581403313156No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA829700.3608634871303911No Hit
TGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC819520.3564358743800146No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC773690.3365029183535161No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA764510.3325102380933534No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG754420.32812176926709613No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATC739610.3216804190870297No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA710230.30890210252454825No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG703340.30590541766697515No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC680980.2961803271857945No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA675980.29400566473472556No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT626020.27227643752364405No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA625800.272180752375797No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCG625660.2721198618271671No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC615710.2677922835495397No Hit
TGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG610170.2653827575537553No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC607500.2642214878048844No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA591930.2574495889322555No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT575240.2501905656705872No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGAT564950.24571511034628712No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT552700.24038718734116807No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC550180.2392911574658293No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG547790.23825166881421833No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG534940.23266278631497098No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC533050.23184076390846692No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC526050.22879623647697028No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC495300.21542206240289588No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCA490850.21348661282144446No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC488820.21260369986631045No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT479850.20870235542909266No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGTAGGCACCATCA464440.20200004575489797No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGATC458540.19943394406263656No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCAC451980.196580786926834No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAGA448090.19488889953990232No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCAT439340.19108324025053156No Hit
GGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATCG434510.1889825163227989No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA431150.18752114315568053No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC422750.1838677102378846No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC405930.17655214575248845No Hit
CGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGC395050.17182008025896228No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAG376960.16395215151099463No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAG373900.16262125809094038No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG371870.16173834513580634No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA369040.1605074861885013No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTGTAGGCACCA365080.15878515352725464No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG349360.1519480147810937No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAGA340180.147955334520931No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG334990.14569803489672137No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA328750.14298405615778725No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG324410.14109644915025935No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA324200.14100511332731444No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA319890.13913055429449298No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT315100.13704722766636884No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG314140.1366296924757636No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAAT310830.1351900659331559No Hit
CGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATCG306260.13320242445287886No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA304610.1324847858440261No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA302850.1317193046612498No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATC297860.12954899153508292No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCCTGTAGGCACCATCAATA297450.12937066921409526No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAGAT293690.12773532305089136No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC292890.12738737705872033No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCAT290140.12619131271063236No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA287870.12520401595784703No Hit
TGCACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGA283010.12309024405540796No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC278200.12099821877747957No Hit
TAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG277660.12076335523276413No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC275650.11988914092743438No Hit
TCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC262790.11429590910328491No Hit
AGTGCGGTAACGCGACCGATCCCGGAGACTGTAGGCACCATCAATAGATC262410.11413063475700365No Hit
GCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCTGTAGGCACCA257630.11205165745378168No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA252080.1096377821330951No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA251560.1094116172381839No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA250800.10908106854562141No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG247670.10771972985125222No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC239740.10427071520385676No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAGA237830.1034399941475484No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATAG230980.10046070658958388No Hit
TGCACGAGCGCACGTGTTAGGACCCGAAACTGTAGGCACCATCAATAGAT230870.10041286401566035No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA230250.1001432058717278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)